摘要
用灭菌棉拭共采集蛋鸭和肉鸭的泄殖腔样品 62只 ,鸡胚尿囊腔传代接种法分离到 1株病毒 ,该病毒可凝集鸡红细胞 ,且不能被 ND、EDS-76阳性血清抑制 ,用病变绒毛尿囊膜制备抗原与禽流感琼扩标准阳性血清作用 ,出现明显的白色沉淀线 ,证明该分离株为 A型流感病毒 ,血凝素亚型分析结果为 H9,电镜负染观察可见典型的禽流感病毒粒子 ,致病性试验结果表明该分离株对鸡表现为弱致病性。研究结果还表明 ,环境 (特别是水体 )
In this study, a strain of virus, which had haemagglutination activity not inhibited by positive sera against newcastle disease virus (NDV) and egg drop syndrome 76 virus (EDSV), was isolated from 62 cloacal swabs of ducks. Agar gel precipitation test and haemagglutination inhibition test indicated the isolate was H 9 subtype of influenza A virus; under electronic microscope, the isolate possessed typical shape of avian influenza virus (AIV); the assay of intravenous pathogenicity showed the isolate had low pathogenicity for chickens; meanwhile, this study implies the water fowl is an important factor and medium for the perpetuation and transmission of avian influenza viruses.
基金
上海市教委资助项目! ( 98EJ0 2 )
