大鼠创伤性脊髓损伤模型的建立

Establishment of traumatic spinal cord injury model in rats

目的:建立一种控制性脊髓挫伤大鼠模型。方法:采用BenchmarkTM立体定位颅脑撞击器制备大鼠创伤性脊髓损伤,并在伤后1、2、3、5、7、142、8 d借助斜板试验及BBB评分评价其运动功能,然后观察损伤部位及其邻近区域的形态学改变。结果:脊髓损伤后大鼠在斜板上维持的角度以及BBB评分显著低于假手术组(P<0.01)。损伤部位可见神经纤维肿胀。灰质部运动神经元肿胀、坏死,尼氏体淡染,甚至溶解。小胶质细胞明显增生。通过铜银染色和Luxol固蓝染色,可见运动神经元溃变以及髓鞘脱失。结论:建立的控制性脊髓挫伤大鼠模型重复性好,适合神经保护药物的药效学筛选。

AIM. To establish a rat model for controlled spinal cord contusion injury. METHODS： Traumatic spinal cord injury model （TSCI） in rats was duplicated by controlled spinal cord contusion iniury method with a BenchmarkTM stereotaxic cortical impactor. Motor func-tion was evaluated at 1, 3, 5, 7, 14, 28 day after TSCI with inclined plane test and BBB score. And then morphological changes were carefully inspected in the injured part and adjacent area. RESULTS： The angle sustaining on the inclined plane and BBB score of spinal cord injured rats were significantly lower than that of sham-operated rats（P〈0.01）. Nerve fibers were swollen in the injured sites. Motoneurons were swollenor loss and Nissl bodies were lightly stained or even dissolved in gray matter. Microglia remark ably proliferated. Motoneuron degeneration and demyelination were observed by using cupric-silver straining and Luxol Fast Blue staining technique. CONCLUSION： The rat model for controlled spinal cord contusion injury is reproducible and suitable for ＂the pharmacodynamic screening of neuroprotective drugs.