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Genetic Analysis of Floating Enteromorpha prolifera in the Yellow Sea with AFLP Marker 被引量:1

Genetic Analysis of Floating Enteromorpha prolifera in the Yellow Sea with AFLP Marker
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摘要 Extremely large accumulation of green algae Enteromorpha prolifera floated along China'coastal region of the Yellow Sea ever since the summer of 2008.Amplified Fragment Length Polymorphism (AFLP) analysis was applied to assess the genetic diversity and relationships among E. prolifera samples collected from 9 affected areas of the Yellow Sea.Two hundred reproducible fragments were generated with 8 AFLP primer combinations,of which 194 (97%) were polymorphic. The average Nei's genetic diversity, the coefficiency of genetic differentiation (Gst), and the average gene flow estimated from Gst in the 9 populations were 0.4018, 0.6404 and 0.2807 respectively. Cluster analysis based on the unweighed pair group method with arithmetic averages (UPGMA) showed that the genetic relationships within one population or among different populations were all related to their collecting locations and sampling time. Large genetic differentiation was detected among the populations.The E. prolifera originated from different areas and were undergoing a course of mixing. Extremely large accumulation of green algae Enteromorpha prolifera floated along China’coastal region of the Yellow Sea ever since the summer of 2008.Amplified Fragment Length Polymorphism (AFLP) analysis was applied to assess the genetic diversity and relationships among E. prolifera samples collected from 9 affected areas of the Yellow Sea.Two hundred reproducible fragments were generated with 8 AFLP primer combinations,of which 194 (97%) were polymorphic. The average Nei’s genetic diversity, the coefficiency of genetic differentiation (Gst), and the average gene flow estimated from Gst in the 9 populations were 0.4018, 0.6404 and 0.2807 respectively. Cluster analysis based on the unweighed pair group method with arithmetic averages (UPGMA) showed that the genetic relationships within one population or among different populations were all related to their collecting locations and sampling time. Large genetic differentiation was detected among the populations.The E. prolifera originated from different areas and were undergoing a course of mixing.
出处 《Journal of Ocean University of China》 SCIE CAS 2011年第3期263-269,共7页 中国海洋大学学报(英文版)
基金 supported by the National Science and Technology Pillar Program(2008BAC-49B01) Qingdao Municipal Science and Technology Plan Project(08-1-7-1-hy),Qingdao Municipal Science and Technology Pillar Plan Project(09-2-5-5-hy)
关键词 AFLP marker Enteromorpha prolifera genetic analysis Yellow Sea AFLP标记 遗传分析 黄海 扩增片段长度多态性 漂浮 算术平均数 遗传多样性 浒苔
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