母乳中溶菌酶含量的初步测定

Preliminary detection of lysozyme content in breast milk

目的:初步研究我国产妇不同时段母乳中溶菌酶含量,为了解我国产妇人群的基本资料奠定基础。方法:采用ELISA法检测溶菌酶含量。结果:母乳中溶菌酶含量在初乳组最高,其次是成熟乳组,过渡乳组最低;其中两两比较:初乳与成熟乳、过渡乳溶菌酶含量比较有统计学差异(P<0.05);成熟乳与过渡乳溶菌酶含量比较无统计学差异(P>0.05);过渡乳组溶菌酶含量与初乳组呈正相关,而与成熟乳组无明显相关性。3组的溶菌酶含量测定值均低于国外报道,尤其是初乳中溶菌酶含量更低。结论:应用ELISA法检测溶菌酶含量具有通量高,所需样本体积量少的优点,将来可以推广应用于大样本人群的调查研究。

Objective：To study the lysozyme content in breast milk of Chinese parturient women at different periods preliminarily,lay a foundation for understanding the basic data of parturient women in China.Methods：ELISA method was used to detect the content of lysozyme.Results：The content of lysozyme in breast milk in colostrum group was the highest,followed by mature milk group,the content of lysozyme in breast milk in transitional milk group was the lowest;there was significant difference in the content of lysozyme in breast milk between colostrum group and mature milk group,transitional milk group（P0.05）;while there was no significant difference in the content of lysozyme in breast milk between mature milk group and transitional milk group（P0.05）;the content of lysozyme in breast milk in transitional milk group was correlated positively with that in colostrum group,but there was no correlation between the content of lysozyme in breast milk in transitional milk group and mature milk group.The contents of lysozyme in breast milk in the three groups were significantly lower than those reported abroad,especially in colostrum group.Conclusion：ELISA has the advantages of high-throughput and low volume of samples in detection of lysozyme content,which can be used for investigation and survey of large population.