摘要
目的:探讨子宫内膜异位症(EMs)在位内膜细胞凋亡特性的改变。方法:①应用免疫组化方法对32例EMs在位子宫内膜组织及24例对照组子宫内膜组织的Survivin蛋白及Ki-67蛋白的表达进行测定;②应用TUNEL技术监测两组子宫内膜细胞的凋亡特性。结果:EMs在位内膜组织Survivin蛋白表达高于对照组,差异有统计学意义(P<0.05);EMs在位内膜细胞分泌期凋亡指数低于同期对照组子宫内膜,差异有统计学意义(P<0.05);EMs在位内膜组织Ki-67蛋白表达与对照组差异无统计学意义(P>0.05)。结论:EMs在位子宫内膜细胞因凋亡能力下降并非高度增殖导致细胞活性升高,在位内膜细胞的生物学特性改变在EMs的病理生理中可能起着重要作用。
Objective：To explore the change of cell apoptosis features of eutopic endometrium in patients with endometriosis.Methods：Immunohistochemical method was used to detect the expression levels of Survivin protein and Ki-67 protein in eutopic endometrium of 32 cases with endometriosis and endometrium of 24 cases in control group;TUNEL technology was used to monitor the features of cell apoptosis of endometrium in the two groups.Results：The expression level of Survivin protein in endometriosis group was significantly higher than that in control group（P0.05）.At secretory phase,the apoptosis index in endometriosis group was significantly lower than that in control group（P0.05）;there was no significant difference in expression level of Ki-67 protein between the two groups（P0.05）.Conclusion：The decrease of ability of cell apoptosis of eutopic endometrium in patients with endometriosis induce the increase of cell activity,the change of biological characteristics of eutopic endometrial cells may play an important role in pathogenesis of endometriosis.
出处
《中国妇幼保健》
CAS
北大核心
2011年第22期3486-3488,共3页
Maternal and Child Health Care of China
