脂膜炎性T细胞淋巴瘤10例临床病理研究

A Clinicopathological Study on Panniculitic T-cell Lymphoma

目的:研究10 例脂膜炎性T细胞淋巴瘤的临床病理形态和生物学行为特点,为临床早期诊断提供依据。方法:运用组织病理学、免疫组化学及分子生物学技术,对10 例原发于皮下脂肪组织中的T细胞淋巴瘤进行临床病理学和免疫组织化学观察及EB 病毒的检测。结果:组织学上以肿瘤细胞(CD45RO阳性)浸润脂肪小叶之间及可见豆袋细胞(CD68 阳性) 为特征,部分患者肿瘤细胞EBV- DNA阳性。2 例患者分别于首诊1 年6 个月和2 年后死亡。结论:脂膜炎性T细胞淋巴瘤多为高度恶性。

Objective:The purpose of the study was to improve the early clinical diagnosis of panniculitic T-cell lymphoma(PTCL) by analyzing the clinical manifestations,pathological features and the biologic behavior in 10 cases of PTCL.Methods:Histopathological method was used to study the morphological characteristics of PTCL. The immunohistochemical determination of LCA, CD20, CD45RO, CD68 in 10 cases of PTCL was carried out using SP or ABC method. Immunohistochemical technique was used to detect Epstein-Barr virus(EBV) antibody, in situ hybridization to detect EBV-RNA and polymerase chain reactive to detect EBV-DNA in 5 cases. Results:Histologically, the lesions were composed of medium sized atypical cells (CD45RO+) infitrating the space between fat cells and some bean bag cells (CD68+) could be observed. Two cases of PTCL were positive by in situ hybridization. One case was positive by PCR. Two patients died within 2 years. Conclusion:PTCL is a type of peripheral T-cell lymphoma with a high malignancy.Pathogenesis of PTCL may be associated with EBV infection.