摘要
目的:建立凤仙花叶片和花朵中香豆素的定性和定量分析方法。方法:以甲醇为提取剂,用索氏提取法提取凤仙花叶片和花朵中香豆素成分,用高效液相色谱法分离和检测(C18柱,紫外检测器,300nm)。结果:凤仙花叶片中香豆素含量为0.1267%,凤仙花花朵中香豆素含量为0.067%。结论:该方法能准确、快速地测定凤仙花叶片和花朵中香豆素的含量。
Objective： To establish a method for the qualitative and quantitative analysis of coumarin compounds in Garden Balsam.Methods： Coumarin compounds in Garden Balsam were extracted with soxhlet extraction using methanol as solvent.The high performance liquid chromatography（C18 column,UV detector,300nm） was used for separation and detection.Results： The content of coumarin in Garden Balsam leaves was 0.1267%,and that in the flowers was 0.067%.Conclusion： The method is accurate,rapid and simple,and is ideal for qualitative and quantitative analysis of coumarin compounds in Garden Balsam.
出处
《分析仪器》
CAS
2011年第4期60-63,共4页
Analytical Instrumentation
