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紫杉醇作用下的HeLa细胞基因表达研究

Studies on the expression of gene in HeLa cell treated with taxol
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摘要 目的;研究HeLa cells在紫杉醇作用下的基因表达。方法:通过生物素-抗生素蛋白DCS体系间接免疫荧光染色法检测Bcl-2及Western blot检测Fas基因的表达。结果;经生物素-抗生素蛋白DCS体系间接免疫荧光染色法检测Bcl-2基因表达加药组荧光强度较阳性对照组明显降低,Western blot法检测Fas基因表达,未见明显条带。 Objective:To study the expression of gene in HeLa cells treated with toxal.Methods:Using indirect immuno-fluorescence staining by biotin-antibiotin protein DCS system detects expression of Bel-2 gene and Western blot detects expression of Fas gene.Result:Indirect immuno-fluorescence staining by biotin-antibiotic protein DCS system detects the low expression of Bcl-2 gene after the cells ware treated with taxol.At the same time Estem blot showed that expression of Fas gene in HeLa cells had no obvious changes.Conclusion:As the cell death occurs the expression of Bcl-2 geng decrease,while the expression of Fas had no obvious changes.
出处 《吉林医学院学报》 1999年第2期11-14,共4页
关键词 HELA CELL 紫杉醇 BCL-2 FAS 药理 HeLa cell taxol Bcl-2 Fas.
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  • 1丁萨姆布鲁克.分子克隆实验指南20,第二版[M].北京:科学出版社,1992..

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