摘要
目的；研究ＨｅＬａ ｃｅｌｌｓ在紫杉醇作用下的基因表达。方法：通过生物素－抗生素蛋白ＤＣＳ体系间接免疫荧光染色法检测Ｂｃｌ－２及Ｗｅｓｔｅｒｎ ｂｌｏｔ检测Ｆａｓ基因的表达。结果；经生物素－抗生素蛋白ＤＣＳ体系间接免疫荧光染色法检测Ｂｃｌ－２基因表达加药组荧光强度较阳性对照组明显降低，Ｗｅｓｔｅｒｎ ｂｌｏｔ法检测Ｆａｓ基因表达，未见明显条带。
Objective:To study the expression of gene in HeLa cells treated with toxal.Methods:Using indirect immuno-fluorescence staining by biotin-antibiotin protein DCS system detects expression of Bel-2 gene and Western blot detects expression of Fas gene.Result:Indirect immuno-fluorescence staining by biotin-antibiotic protein DCS system detects the low expression of Bcl-2 gene after the cells ware treated with taxol.At the same time Estem blot showed that expression of Fas gene in HeLa cells had no obvious changes.Conclusion:As the cell death occurs the expression of Bcl-2 geng decrease,while the expression of Fas had no obvious changes.
