STAT3反义寡核苷酸纳米复合微粒的制备及特性研究

Preparation and characterization of polyamidoamine dendrimer-STAT3-antisense oligodeoxynucleotides nanoparticles

【目的】研究STAT3反义寡核苷酸纳米复合微粒的制备工艺，并对纳米复合微粒特性进行评价。【方法】将纳米技术与反义寡核苷酸技术相结合，制备STAT3反义寡核苷酸纳米复合微粒，并观察纳米微粒对限制性内切酶的抗性及其在不同pH值下的稳定性。【结果】将纳米技术与反义寡核苷酸技术相结合，制备STAT3反义寡核苷酸纳米复合微粒，并观察纳米微粒对限制性内切酶的抗性及其在不同pH值下的稳定性。【结果】在pH值为2～10的范围内，PAMAM—STAT3一asODN纳米微粒均未出现降解，且该纳米微粒具有较好的抗限制性内切酶的特性。【结论】新型STAT3反义寡核苷酸纳米微粒具有较好的抗限制性内切酶、耐酸碱的特性，为其在基因治疗中的应用提供了一定的依据。

[Objective] To study the technique for the preparation of polyamidoamine dendrimer-STAT3-antisense oligodeoxy-nucleotides nanoparticles （PAMAM- STAT3- asODN- NPs）, and to evaluate the characteristics of the prepared nanoparticles. [ Methods] The PAMAM- STAT3- asODN- NP was Prepared with nanotechnology and antisense oligonucteotide .technology, and the resistance of nanoparticles to restriction enzyme and the stability of nanoparticles in different PH value were observed. [ Results] PAMAM- STAT3- asODN- NPs were not degradated in the range of pH2 to pill0, and had better resistance to restriction enzyme. [Conclusion] The nanoparticles has the characteristics of resisting restriction enzyme, acid-resistant and alkali-resistant. This experiment provides a basis for the utilization of the nanoparticles in gene therapy.