铁皮石斛对人正常皮肤成纤维细胞GM0639辐照后微核的影响研究

The Effect ofDendrobium candidum Wall. ex Lindl Additive Combined with Irradiation on Micronucleus of GM0639 Cells

目的分析铁皮石斛煎剂作用后人正常皮肤成纤维细胞GM0639微核的变化。方法通过MTT法测定不同药物浓度及不同药物作用时间对GM0639细胞存活率的影响，MTT法测定相同剂量^60Coγ射线辐照后培养24h未加药组和加不同浓度药物组细胞的存活率，通过胞质分裂阻滞法（CB法）计数铁皮石斛煎剂作用联合不同剂量^60Coγ射线照射后的微核率（Micronucleus frequency，MNF）和微核细胞率（Micronucleus cell frequency，MNCF），将铁皮石斛煎剂作用的GM0639细胞与单纯照射细胞的微核率和微核细胞率的统计分析结果进行比较。结果铁皮石斛煎剂在给药浓度0-100mg／mL范围内，铁皮石斛对GM0639细胞没有细胞毒性。4Gy照射后培养24h，随着药物浓度的增加，细胞的存活率均显著增加，加药60mg／mL就可明显提高细胞的存活率。4Gy照射后培养24h，在试验药物浓度为60mg／mL，作用时间为24h时，将CB微核法得到的数据进行回归拟合可以得到药物作用及未经药物作用的剂量效应曲线，比较两组曲线，铁皮石斛作用组的微核率及微核细胞率明显低于单纯照射组（P〈0．05）。结论铁皮石斛可以降低辐照后GM063细胞微核率及微核细胞率，铁皮石斛具有较好的抗辐射作用。

To analyze the change of micronucleus in GM0639 Cells caused by Dendrobium candidum Wall. ex Lindl（D. officinale） additive,MTT method was used to check the effect of different concentration and treatment time of D. officinale to cytotoxicity.Cytokinesis block micronucleus method（CB method） was used to determine the micronucleus frequency（MNF） and the micronucleus cell frequency（MNCF） of cells treated by D. officinale additive and with different doses of ^60Co 7 -rays.The experiment results show D. officinale has no cytotoxicity in the range of 0 -100mg/mL to GM0639 Cells.When drug concentration is 60mg/mL and treatment time is 24h,the dose-response curves of MNF and MNCF of irradiated cells combined with treatment of or not D. officinale additive confirm that MNF and MNCF of cells treated by D. officinale are both significantly lower than that treated by radiation alone.The cell experiments demonstrated that D. officinale has radiation protective effects on GM0639 Cells.