三种不同检测方法对婴幼儿丙型肝炎病毒感染的诊断价值

Value of three methods in diagnosis of hepatitis C virus infection in infants

目的探讨不同检测方法对婴幼儿丙型肝炎病毒感染诊断结果的影响。方法对66例ELISA法检测抗HCV阳性孕妇所生婴幼儿血清,分别用ELISA法检测抗HCV和HCV-cAg,用荧光定量PCR法检测HCV-RNA,并对结果对比分析。结果 66例婴幼儿血清中,61例抗HCV阳性,阳性率92.4%;9例HCV-cAg阳性,阳性率13.6%;11例HCV-RNA阳性,阳性率16.6%。结论抗HCV检测阳性率过高,且有可能漏诊。建议采用HCV-RNA检测和HCV-cAg综合诊断婴幼儿HCV感染。

Objective To investigate the effect of different methods in diagnosis of hepatitis C virus infection in infants. Methods Serum was separated from 66 infants whose mother＇s HCV was positive. HCV and HCV-cAg were measured by ELISA. HCV-RNA was detected by Real-time quantitative PCR. Results HCV was positive in 61 ser um samples, HCV-cAg was positive in 9 serum samples（13.6 %）, while HCV-RNA was positive in 11 serum sampies（16.6%）. Conehtsions The high positive rate of HCV may cause missed diagnosis, so HCV-RNA and HCVeAg should be combined together to detect in the evaluation of hepatitis C virus infection in infants.