小儿泻痢片定性定量研究

Quantitative and qualitative study of Xiao'er Xieli Pian

目的建立小儿泻痢片定性定量研究方法。方法采用TLC法对葛根、黄连进行定性鉴别;采用HPLC法测定小儿泻痢片中黄芩苷的含量,选用Diamonsil C18（250 mm×4.6 mm,5μm）色谱柱,流动相为甲醇-水-磷酸（47∶53∶0.2）,流速：1.0 mL·min-1,柱温30℃,检测波长280 nm。结果 TLC检出了葛根、黄连特征斑点,阴性无干扰;黄芩苷在0.0750.60μg与峰面积线性关系良好（r=0.999 6）;加样回收率为98.0%,RSD=1.9%。结论方法简便、稳定,可作为本品的定性定量方法。

Objective To establish a quantitative and qualitative method for Xiao’er Xieli Pian.Methods Radix Puerariae and Rhizoma Coptidis were identified by TLC in Xiao’er Xieli Pian.The content of baicalin was determined by HPLC.The chromatographic column was Diamonsil C18 Column（250 mn×4.6 mm,5 μm）.The mobile phase consisted of ethanol-water-sulfuric acid（47∶53∶0.2）.The flow rate was 1.0 mL·min-1,the column temperature was 30 ℃,and the wavelength was 280 nm.Results TLC quality identification detected Radix Puerariae and Rhizoma Coptidis.Baicalin was in good linearity（r=0.999 6） within 0.075-0.60 μg.The average recovery was 98.0% with RSD=1.9%.Conclusion The method is simple,stable,and can be used for the quality control of Xiao’er Xieli Pian.