摘要
目的:研究MK801对大鼠脑缺血再灌注后海马CA1区c-fos基因表达及神经元凋亡的影响。方法:将大鼠随机分为正常对照组、脑缺血再灌注模型组和脑缺血用药后再灌注组,用药组在再灌注前经腹腔注射MK801(0.5mg/kg);分别于再灌注后2、6、24和48 h,采用免疫组化法和Western印迹观察海马CA1区c-fos基因的表达情况。结果:c-fos基因的表达在脑缺血再灌注后2 h即开始增强,至再灌注后6 h达到高峰,24 h表达降低,至48 h又至高峰,但较再灌注6 h后较低。用药组海马CA1区c-fos基因的表达均较模型组的相应时间段降低,具有显著性差异(P<0.01)。结论:海马CA1区c-fos基因在脑缺血再灌注后表达升高,MK801可降低脑缺血再灌注后c-fos基因的表达,对缺血再灌注后的脑组织具有保护作用。
Objective： To observe the effect of MK801 on the expression of c-fos gene and its protective effects on the nerves in the hippocampus CA1 region of the rats after the brain ischemic-reperfussion injury.Methods： The study rats were divided into the control group,the ischemic-reperfussion model group,and the MK801 treated（0.5 mg/kg,i.v.） group.The expression of c-fos gene in the hippocampus CA1 region was measured by immunohistochemistry and Western blotting methods post ischemic-reperfussion at 2,6,24 and 48 h point.Results： The expression of the c-fos gene appeared at 2 h after the brain ischemic-reperfussion and it was up to its maximum 4 h later,then it decreased at 24 h point,at 48 h point it was back up,but lower than the highest point.Compared with the model group,the expression of the c-fos gene was lower than MK801 group at the same time interval.There were statistically significant difference between them（P0.01）.Conclusion： MK801 can decrease the expression of the c-fos gene and has the protective effects on the nerves in the hippocampus CA1 region after the ischemic-reperfussion injury.
出处
《生物技术通讯》
CAS
2011年第4期532-535,共4页
Letters in Biotechnology
