Caveolin-1在慢性肝炎、肝硬化和肝癌组织中的表达

Caveolin-1 expression in chronic viral hepatitis,developing cirrhosis and hepatocellular carcinoma

目的:Caveolin-1作为候选抑癌基因,在多种肿瘤均有异常表达,本研究探讨Caveolin-1在乙型肝炎、肝硬化和肝癌组织的表达特点。方法:采用免疫组织化学(immunohistochemistry,IHC)染色,在完全相同的实验条件下检测22例乙型肝炎、16例肝硬化和11例肝癌组织中Caveolin-1的表达状况。结果:免疫组化结果显示Caveolin-1在乙型肝炎、肝硬化和肝癌组织的阳性率分别为77.3%、68.8%和27.3%;肝癌与其它两组间(慢性肝炎、肝硬化)的阳性率有显著性差异(x2=-2.868,P=0.007;x2=-2.296,P=0.03)。肝硬化较肝炎的Caveolin-1阳性率低,但无统计学意义(x2=-0.512,P=0.612)。结论:Caveolin-1在肝细胞中的表达水平随着肝癌的发生呈进行性下调乃至缺失。

Objective：Caveolin-1,a candidate tumor suppressor gene,aberrantly expressed in many kinds of carcinomas.This research was designed to check the expression of Caveolin-1 in chronic viral hepatitis,developing cirrhosis and liver carcinoma.Methods：Immunohistochemistry（IHC） was used to examine the expression of Caveolin-1 in 22 specimens of chronic viral hepatitis,16 specimens of developing cirrhosis and 11 specimens of liver carcinoma.Results： Positive rate of caveolin-1 in 11 specimens of liver carcinoma than in chronic viral hepatitis and developing cirrhosis（27.3%vs.77.3%,66.8%,P〈0.05）.Positive rate of caveolin-1 was lower in developing cirrhosis than in chronic viral hepatitis,but the difference was not significant（P〉0.05）.Conclusions： Progressive down-regulation of caveolin-1 in liver tissue is correlated to hepatic carcinogenesis.