高效液相色谱法测定风柜斗草中槲皮素和异鼠李素的含量

Determination of Quercetin and Isorhamnetin in the Medicinal Herb of Sarcopyramis Nepalensis Wall by HPLC

目的:建立闽南草药风柜斗草中槲皮素和异鼠李素含量测定的方法。方法:采用70%甲醇超声提取,HPLC-PDA分析方法测定。色谱条件为色谱柱Ultimate Column TMC18 4.6×150mm×5μm,流动相甲醇∶0.5%磷酸=55∶45,流速:1.0 mL/min,柱温:30℃,检测波长:370nm。结果:闽南草药风柜斗草中槲皮素在10～200μg/mL与峰面积的线性关系良好,回归方程为:Y=38409X-54808(r=0.9998),平均回收率为95.2%,RSD值为0.72%;异鼠李素在5～101μg/mL与峰面积的线性关系良好,回归方程为:Y=37623X-26662(r=0.9997),平均回收率为97.03%,RSD为2.35%。结论:本试验建立的HPLC-PDA分析方法具有回收率高、重现性好、选择性强等特点,适用于闽南草药风柜斗草中槲皮素和异鼠李素含量的测定。

Objective：To establish a method for determining the content of quercetin and isorhamnetin in the medicinal herb of Sarcopyramis nepalensis Wall.Methods：With absolute methanol solvent,ultrasonic extraction,UltimateTM C18（4.6＊150mm,5μm） column,methanol：0.5% phosphoric acid = 55：45（V / V） as mobile phase,the flow rate was 1.0ml/min,The column temperature was at 30℃ with 370nm detection wavelength,HPLC-PDA determination of quercetin.Results：The linear range of quercetin concentration was from 10μg/mL to 200μg/mL,Y=38409X-54808（r=0.9998）,The average recovery was 95.2% with the RSD of 0.72%.The linear range of isorhamnetin concentration was from 5μg/mL to 101μg/mL,Y=37623X-26662（r=0.9997）,The average recovery was 97.03% with the RSD of 2.35%.Conclusion：The proposed method had highly repeatability and selectivity.It was simple,fast and could be used for the quality control of quercetin and isorhamnetin in the medicinal herb of Sarcopyramis nepalensis Wall.