酶联免疫定量测试盒法测定黄曲霉毒素B_1的方法探讨

Discussion about the Determination of Aflatoxin B1 by ELISA and the Quantitative Test Kit Method

本文主要通过pH值、阳离子(如Cu2+、Fe3+等)、溶液呈色(如红曲)三方面来探讨酶联免疫测试盒法测定黄曲霉毒素B1时,用甲醇水溶液提取样品中黄曲霉毒素B1的提取方法易出现假阳性,干扰测定,必须进一步用三氯甲烷来提取黄曲霉毒素B1以消除假阳性、排除干扰,提高测定数据的准确性。

This article was studied pH、cations（such as Cu2＋,Fe3＋,etc.）、coloring solution（such as red yeast） to explore the three ELISA test kit method to measure Aflatoxin B1 samples when the extraction of aflatoxin B1 Extracted with methanol solution method prone to false positive,interfered with the determination to be further used to extract aflatoxin B1 chloroform to eliminate false positives,eliminate interference and improve the measurement accuracy of the data.