zebularine对卵巢癌细胞的生长抑制作用

Effect of zebularine on proliferation of human ovarian cancer cell line and its mechanism

目的：观察去甲基化制剂zebularine（Zeb）对体外培养的卵巢癌细胞A2780的生长抑制作用,并进一步探讨其作用机制。方法：应用不同浓度（50、100、200μmo.lL-1）的Zeb处理体外培养的卵巢癌细胞A2780;应用MTT法检测细胞增殖活性,流式细胞仪检测细胞周期分布,Annexin V-FITC染色法检测细胞凋亡率。结果：100、200μmo.lL-1的Zeb处理卵巢癌细胞A2780 24~72 h后,细胞增殖显著受到抑制（P〈0.05）;100、200μmo.lL-1的Zeb处理72 h后A2780细胞G2期细胞数量显著增加（P〈0.05）,S期细胞数显著减少（P〈0.05）;100、200μmo.lL-1组细胞凋亡率均增加（P〈0.05）。结论：Zeb对卵巢癌细胞A2780具有生长抑制作用,其机制与阻滞细胞周期及诱导凋亡有关。

Objective： To investigate the effect of zebularine（Zeb）,one of the demethylating agents,on the proliferation of human ovarian cancer cell line A2780 and its mechanism.Methods： A2780 cells cultured in RPMI 1640 were repectively treated with 50,100,and 200 μmol·L-1 of Zeb.MTT assay,flow cytometry and Annexin V-FITC staining were performed to analyze the cell proliferation activity,cell cycle and apoptotic rate.Results： After A2780 cells were treated with Zeb of 100 and 200 μmol·L-1 for 24 to 72 hours,the cell proliferation activity was inhibited（P0.05）.The number of cells in G2 phase increased and the number of cells in S phase decreased significantly after the cells were treated with Zeb of 100 and 200 μmol·L-1 for 72 hours（P0.05）.The apoptotic rate increased significantly in cells treated with Zeb of 100 and 200 μmol·L-1（P0.05）.Conclusion： Zeb could inhibit the growth of human ovarian cancer cell A2780,possibly via blocking cell cycle and inducing apoptosis.