MG132和DDP联合应用诱导Hep-2喉癌细胞凋亡及机制的研究

Apoptosis and machanism of laryngeal carcinoma cell Hep-2 treated with combined MG132 and DDP

目的:研究蛋白酶体抑制剂MG132与顺铂(DDP)联合应用对Hep-2喉癌细胞凋亡的影响,同时观察细胞中凋亡相关蛋白Bcl-2、Bad表达水平的变化。方法:体外培养Hep-2细胞分别暴露于MG132、DDP或者MG132和DDP,24h后流式细胞术(FCW)检测Hep-2细胞的凋亡率,Western blot方法测定Hep-2细胞中Bcl-2和Bad两种蛋白的表达情况。结果:流式细胞术检测结果显示,MG132和DDP联合用药24h后Hep-2细胞凋亡率较单独应用MG132、DDP显著增加。Western blot结果显示联合用药组较单独用药组Hep-2细胞中Bcl-2的表达显著减少,而Bad的表达显著增加。结论:MG132联合DDP可能通过降低Bcl-2同时增强Bad的表达而进一步诱导Hep-2细胞的凋亡。可以认为MG132能增强DDP诱导Hep-2细胞凋亡的作用。

Objective：To study the apoptosis of laryngeal carcinoma cell Hep-2 treated with proteasomes inhibitor MG132 associated with DDP,and to observe the expression of Bcl-2 and Bad in Hep-2 cells.Methods： Hep-2 cells were exposed to MG132,DDP or MG132 and DDP in vitro,24 later,flow cytometry was used to detect the apoptosis of Hep-2 cells,and the expressoin of Bcl-2 and Bad in Hep-2 cells was measured with Western blot.Results： The apoptosis of Hep-2 cells treated with MG132 and DDP simultaneously was increased significantly than alone exposed to MG132 or DDP.Meanwhile,the expression of Bcl-2 was decreased,and the expression of Bad was increased in Hep-2 cells.Conclusion： MG132 associated with DDP could further induce Hep-2 cells apoptosis through depressing the expression of Bcl-2 and promoting Bad expression.MG132 could enhance the effect of DDP to Hep-2 cells apoptosis.