不同浓度哇巴因对A549细胞增殖及死亡的影响

Influence of different concentration of ouabain on the proliferation and death of A549 cells

目的:评价特异性钠泵抑制剂哇巴因在体外对非小细胞肺癌A549细胞增殖与死亡的影响。方法:利用细胞培养技术,采用MTT法、流式细胞技术、TUNEL法及电镜技术分别评价不同浓度哇巴因对A549增殖与死亡的影响。结果:当哇巴因浓度<1.8nmol/L时,可通过干扰细胞周期,显示出促进肿瘤细胞增殖作用。哇巴因浓度≥1.8nmol/L时,肿瘤细胞增殖受抑。哇巴因对A549细胞增殖的抑制作用呈明显的剂量依赖性和时间依赖性。高浓度哇巴因(≥100nmol/L)所致的A549细胞死亡以非凋亡性死亡为主。结论:哇巴因在适当浓度具有抗肺肿瘤活性,可能成为潜在的抗肺肿瘤药物。

Objective：To evaluate the effect of specific sodium pump inhibitor ouabain on the proliferation and death of A549 cells in vitro.Methods： Using MTT,flow cytomertry,TUNEL and electron microscopy to evaluate different concentration of ouabain on the proliferation and death of cultured A549 cells.Results： When concentration of ouabain was 1.8nmol/L,it stimulated the growth of cancer cells through interference of cells cycle.When concentration of ouabain was≥1.8nmol/L,proliferation of cancer cells was inhibited in time-dependent and dose-dependent way.When concentration was ≥100nmol/L,ouabain induced a non-apoptotic-related cells death process.Conclusion： Ouabain has anti-lung cancer effects in proper concentration,and may be a potential anticancer drug.