Survivin在人胃腺癌中的表达及其与临床病理的关系

Expression of Survivin and its clinical significance in human gastric adenocarcinoma

目的:研究Survivin蛋白在人胃腺癌细胞系和组织中的表达及其临床意义。方法:用RT-PCR、Western Blot方法及免疫组织化学方法检测6株人胃腺癌细胞系,80例人胃腺癌及癌旁组织中Survivin基因的表达。结果:Survivin在6株人胃腺癌细胞系均有表达,胃腺癌组织中Survivin表达阳性率为60%,癌旁正常胃组织均无Survivin的表达;在胃腺癌中的表达与患者生存期和肿瘤的区域淋巴结转移(P<0.05)密切相关,与肿瘤分化程度无相关性(P>0.05)。结论:Survivin的变化与胃腺癌的发生和预后密切相关,并可能成为一个有希望的肿瘤治疗靶点。

Objective：To investigate the expression of Survivin in human gastric adenocarcinoma and analyze its relationship with clinicopathological parameters.Methods： The expression of Survivin expression was assessed using RT-PCR and western blot and immunohistochemistry（IHC） in 6 human gastric adenocarcinoma cell lines and 80 human gastric adenocarcinoma tissues and their corresponding normal tissues.The relationship between Survivin expression and the clinicopathological factors was analyzed.Results： Survivin was expressed in all 6 haman gastric carcinoma cell lines.The positive rates of Survivin protein expression was 60% in gastric adenocarcinoma tissue and not found in normal gastric mucosa respectively.Survivin protein was correlated with survival and lymph node metastasis（P0.05）,but had no obvious relationship with differentiation status and stage（P0.05）.Conclusion： The change of Survivin expression may be involved maily in gastric carcinogenesis and prognosis of gastric adenocarcinoma.Survivin would be a hopeful target of gastric carcinoma therapy.