STAT3与Survivin在宫颈癌组织中的表达及意义

Expression and significance of STAT3 and Survivin in cervical cancer

目的:研究信号转导与转录调控因子3(STAT3)和存活素(Survivin)在宫颈癌及正常宫颈组织中的表达,探讨其与宫颈癌的发生、发展、转移的关系。方法:采用链霉素抗生物素蛋白-过氧化物酶(SP)法检测30例宫颈癌组织、10例宫颈上皮内瘤样病变组织(CIN)和10例正常宫颈组织中STAT3和Survivin的表达,并应用自动图像分析仪定量分析STAT3和Survivin在宫颈癌组织中表达的相对含量(灰度)。结果:STAT3在正常宫颈组织中的阳性表达率为10%,在CIN组织中的阳性表达率为30%,差异无显著性(P>0.05),但有升高趋势。STAT3在宫颈癌组织中的阳性表达率为73.33%,与正常宫颈组织相比,差异有显著性(P<0.05),与CIN组织相比,差异无显著性(P>0.05)。Survivin在正常宫颈组织中的阳性表达率为0%,在CIN组织中的阳性表达率为40.00%,两者相比,差异有显著性(P<0.05)。Survivin在宫颈癌组织中的阳性表达率为86.67%,与正常宫颈组织相比,差异有显著性(P<0.05);与CIN组织相比,差异有显著性(P<0.05)。STAT3和Survivin的平均灰度值均与宫颈癌的临床分期、组织分化和淋巴结转移显著相关(P<0.05)。STAT3及Survivin在宫颈癌组织中的表达亦有明显的相关性(P<0.05)。结论:STAT3和Survivin与宫颈癌的恶性程度、进展及转移有关,且两者之间有明显的相关性,表明宫颈癌的发生发展可能与STAT3信号传导通路激活,细胞凋亡受抑制有关。

Objective：To investige the expression of STAT3 and Survivin in cervical cancer,and discuss the relationship between their expression and the genesis,development and prognosis of cervical cancer.Methods： The expressrions of STAT3 and Survivin were detected using immunohistochemical SP technique in the tissues of normal cervical epithelium,cervical intraepithelial neoplasia（CIN） and cervical cancer.Meanwile,the gray scale of STAT3 and Survivin expression in cervical cancer was analyzed by automatic image analyzer.Results：The positive expression of STAT3 was detected in 10.00% of normal cervical epithelium and in 30.00% of CIN.Though there was a rising tendency of positive expression rate of STAT3 in these two groups,the difference was not significant（P0.05）.However,the positive expression rate in cervical cancer was 73.33%,which was signicantly higher than that in normal cervical epithelium（P0.05）.Yet the difference was not significantly important between cervical cancer and CIN（P0.05）.The positive expression of Survivin was not detected in normal cervical epithelium,but was detected in 40.00% of CIN and 86.67% of cervical cancer.The difference of positive expression rate of Survivin in normal cervical epithelium and CIN was significant（P0.05）,and also was in cervical cancer,normal cervical epithelium（P0.05）,cervical cancer,CIN（P0.05）.The average values of gray scale of both STAT3 and Survivin were signicantly correlated with clinical stage,differentiation degree and lymph node metastasis of cervical cancer（P0.05）.Conclusion：STAT3 and Survivin were related with malignant grade,progression and metastasis of cervical cancer.In addition,the statistically significant correlation of the expression of STAT3 with Survivin indicated that the occurrence and development of cervical cancer might be associated with the activation of STAT3 signal transduction pathways and inhibition of cell apoptosis.