不同溶剂抽提冷杉脂溶性物质的GC/MS分析

Component Analysis of Lipid-Soluble Substances Extracted by Different Solvents from Fir by GC/MS

为了研究不同溶剂超声波抽提的冷杉脂溶性物质中有机化学成分的异同,采用氯仿一元溶剂、V(氯仿)∶V(甲醇)=3∶1的混合二元溶剂以及V(氯仿)∶V(甲醇)∶V(丙酮)=4∶1∶1的混合三元溶剂,超声波抽提冷杉脂溶性物质的化学成分,气相色谱法分离,质谱法鉴定其结构。结果表明,3种不同溶剂所抽提的脂溶性物质在提取率、化学成分及含量等方面存在较大的差异。用氯仿一元溶剂超声波抽提的脂溶性物质的提取率最高,化学成分比较全面,是冷杉脂溶性物质抽提的理想溶剂。

The chemical components of lipid-soluble substances from Fir were extracted by ultrasonic extraction with the single solvent（chloroform）,the binary solvents（V（chloroform）∶V（methanol）=3∶1） and the ternary solvents（V（chloroform）∶V（methanol）∶V（acetone）=4∶1∶1） respectively,separated and identified by gas chromatography-mass spectrometry（GC/MS）.The results show that the extraction rate,composition and their contents of lipid-solvent substances are notably varied by different solvents.The single solvent（chloroform） for extracting lipid-solvent substances is better than the other in raising yield and chemical composition,which is an ideal solvent for extracting of lipid soluble substances from Fir.