高效液相法测定牡丹皮药材中丹皮酚的含量

Determination of Paeonol in Cortex Moutan by HPLC

用高效液相色谱法测定5个产地丹皮药材的丹皮酚含量。色谱柱为菲罗门LunaC18柱(5μm,4.6mm×250mm),流动相为甲醇—水(45∶55),流速1mL/min,检测波长274nm,柱温25℃。结果表明,丹皮酚在0.432—4.32μg线性良好,相关系数r=0.9997,平均回收率为100.08%,RSD为2.85%(n=5)。丹皮酚含量按其平均值为安徽丹皮>陕西丹皮>山东丹皮>河南丹皮>重庆丹皮。该方法快速简便,结果准确可靠。

To determine paeonol in cortex moutan from five areas by HPLC.HPLC column was a Phenomenex Luna C18（5μm,4.6mm×250 mm）,with mobile phase of methanol-water（45∶55） and the flow rate at 1 mL/min.The detective wavelength was 274 nm and the column temperature was 25℃.The results showed that the linear of paeonol was good in the range of 0.432μg—4.32 μg（r=0.9997）,and the average recovery was 100.08%（RSD=2.85%）.The average content of paeonol in cortex moutan was： AnhuiShanxiShandongHenanChongqing.This method was rapid and convenient,results were correct and reliable.