三种方法提取不同品种梨叶片总RNA

Three Methods for Extracting Total RNA from Leaves of Pear Varieties

为筛选出提取梨叶片总RNA的最佳方法,以梨极矮化突变体和苹果梨为材料,采用柱式植物RNAout试剂盒、SDS法和改良CTAB法提取其叶片总RNA并比较提取效果。结果表明,改良CTAB法能有效抑制酚类物质和多糖对总RNA提取的影响,获得纯度高、完整性好的总RNA,其28 S rRNA条带亮度高于18 S rRNA,所提取的RNA适合下一步的研究使用。

In order to choose the best method for extracting total RNA from pear leaves,the effects of Column Plant RNAout,SDS method,and modified CTAB method were compared by extracting total RNA from the leaves of pear dwarf mutant and Pingguoli.The results showed that the modified CTAB method was more suitable as it could eliminate the pollution of polyphenol and polysaccharide and got high quality of RNA.The brightness of the brand of 28S rRNA was higher than that of 18S rRNA.And the total RNA was suitable for further research.