维吾尔族妇女宫颈病变病理进程与降钙素相关肽α表达的关系

The association of cervical lesion pathogenesis in Uighur women with the protein expression of calcitoninrelated polypeptide alpha

目的:观察降钙素相关肽α(calcitonin-related polypeptide alpha,CALCA)的蛋白表达水平与宫颈病变病理进程的关系。方法:收集维吾尔族妇女宫颈炎、低度鳞状上皮内瘤变(LSIL)、高度鳞状上皮内瘤变(HSIL)和宫颈鳞癌(CSCC)患者的石蜡包埋组织标本共104例,采用免疫组织化学方法检测CALCA蛋白的表达水平。结果:CALCA蛋白在大部分宫颈炎患者的宫颈上皮细胞中强表达,但是随着宫颈上皮内瘤变和宫颈癌的发生,其表达水平呈现从强表达到弱表达或表达缺失的趋势改变,且与宫颈病变病理进程呈负相关(r=-0.361,P<0.01),其中宫颈炎、LSIL、HSIL和CSCC组患者的CALCA蛋白表达缺失率分别为45.5%(15/33)、75%(12/16)、80.8%(21/26)和82.8%(24/29),C ALCA蛋白表达缺失率在HSIL及CSCC与宫颈炎间的差异具有统计学意义(P<0.01),而在LSIL与宫颈炎间的差异无统计学意义(P>0.05)。结论:CALCA蛋白表达缺失与维吾尔族妇女宫颈癌前病变及癌症的发生有一定的关系。

OBJECTIVE：To investigate the association of calcitonin-related polypeptide alpha（CALCA） protein expression level with cervical lesion pathogenesis.METHODS：We collected 104 cases of paraffin-embedded cervical tissue specimens of Uighur women with cervicitis,low grade squamous intraepithelial lesion（LSIL）,high grade squamous intraepithelial lesion（HSIL） and cervical squamous cell carcinoma（CSCC）,and evaluated the CALCA protein expression by immunohistochemistry.RESULTS：CALCA protein was strongly expressed by cervical epithelial cells in most cases of cervicitis,but the expression level changed from strong to weak or loss of expressions with the development of cervical intraepithelial neoplasia and cervical carcinoma.The rates of CALCA protein expression loss in the groups of cervicitis,LSIL,HSIL and CSCC were 45.5%（15/33）,75%（12/16）,80.8%（21/26）and 82.8%（24/29）,respectively. The expression level was negatively correlated with cervical lesion pathogenesis（r = -0.361,P0.01）,and the difference was statistically significant for HSIL and CSCC compared to cervicitis（P0.01）,but not for LSIL（P0.05）. CONCLUSION：This study showed that the loss of CALCA protein expression was to some extent,associated with the development of cervical pre-cancerous lesions and cancer.