黄芪多糖对接种NDⅣ雏鸡红细胞免疫功能的影响

Effects of APS on the immune function of erythrocyte in chicken inoculated with NDⅣ vaccine

以50日龄内健康海兰白雏鸡为研究对象,将黄芪多糖(APS)按0 mg/只、5 mg/只和10 mg/只经滴鼻点眼、肌肉注射等方式分别与鸡NDⅣ系疫苗同时免疫接种适龄雏鸡。结果表明,雏鸡C3b受体花环率(E-C3bRR)和红细胞C3b受体花环促进率(ERER)显著升高,红细胞免疫复合物花环率(E-ICRR)和红细胞C3b受体花环抑制率(ERIR)则降低,即APS可显著提高雏鸡的红细胞免疫功能,且APS 10 mg/只组效果优于5 mg/只组;对NDⅣ系苗免疫雏鸡来说,以APS 10 mg/只肌肉注射,NDⅣ滴鼻点眼对红细胞免疫功能的增强作用最显著。

The clinical healthy Hyaline white chickens aged 1-50 days were randomly divided into different groups and vaccinated with NDⅣ vaccine.Different dosage of APS（0,5 and 10 mg every bird） were used as immunoadjuvant to each group by dropping lotion in eyes and noses or intramuscular injection with vaccine respectively.The results were as follows：①APS as immunoadjuvant could significantly increase E-C3bRR and ERER,and decrease E-ICRR and ERIR.It meant that APS could enhance the immune function of chicken erythrocytes,and the dosage of 10 mg was better than 5 mg.②Intramuscular APS injection（10 mg） combined with NDⅣ vaccination by dropping lotion in eyes and noses could provide the optimal effect on the immune function of chicken erythrocytes.