猪伪狂犬病病毒的分离鉴定

The Separation and Identification of Pig Pseudo Hydrophobic Virus

从山西某猪场发病猪的脑组织中分离到1株疑为猪伪狂犬病病毒(PRV)毒株,通过BHK-21细胞培养、病毒毒力滴定、中和试验、PCR检测病毒方法对该分离株进行了鉴定,结果发现:分离的病毒在BHK-21细胞培养盲传4代后出现典型细胞病变;用BHK-21细胞测定其毒价TCID50为10-8.042/0.1 mL;PCR体外扩增可见其扩增片段与预期目的条带相一致;据此分离病毒株的上述特征,鉴定该病毒为猪伪狂犬病病毒,并命名为SX09。

We separated a suspected pseudo rabies virus（PRV） strain from some sick pigs＇ brain tissue in a Shanxi piggery,and identified it by the BHK-21 cell culture,virus toxic titration,neutralization test,and PCR detection method of the virus.The results were that： The separated virus had typical cytopathy in BHK-21 cell culture blind passage after four generations;Using BHK-21 cells determination,the poison price of TCID50 10-8.042/0.1 mL was got;By polymerase chain reaction（PCR） expand in vitro could see its amplification clips in line with the expected purpose segment strip;According to the above characteristics of separated strains,the pig pseudo hydrophobic virus was identified and named as SX09.