荧光辅助糖电泳检测葡甘露寡糖条件的分析

Analysis of Glucomanno-Oligosaccharides by Fluorophore-Assisted Carbohydrate Electrophoresis

【目的】探索荧光辅助糖电泳(FACE)检测葡甘露寡糖组分的条件。【方法】以魔芋葡甘露寡糖为原料,对比3种荧光衍生剂的电泳分离效果,在此基础上研究分离胶浓度、衍生化反应条件及样品杂质对FACE分离效果的影响,确定FACE分析葡甘露寡糖的最佳条件。【结果】FACE分析葡甘露寡糖最佳条件是:荧光衍生剂为ANTS,分离胶浓度25%,ANTS用量2μL,衍生化反应温度37℃,衍生化反应时间16 h。【结论】FACE对葡甘露寡糖组分有很好的分离效果,使用本方法可以确定葡甘露寡糖中至少含有14种组分,为葡甘露寡糖的定性定量分析提供一种有效的检测手段。

[ Objective] The objective of this study was to explore the conditions of FACE detecting glucomanno- oligosaccharides. [Method] Konjac glucomanno-oligosaccharides was used as the substrate, the optimal parameters were determined by investigating the effects of 3 kinds of fluorescer, the concentration of resolving gel, the conditions of derivatization reaction and sample impurities on FACE. [Result] The optimal conditions of FACE used in detecting glucomanno-oligosaccharides were as follows： fluorescer ANTS, concentration of resolving gel 25%, content of ANTS 2 BL, derivation reaction temperature 37 ℃ and derivation reaction time 16 h. [Conclusion] These findings show that FACE has a high resolution to glucomannooligosaccharides, and 14 kinds of components were detected by FACE, which provides an effective method for qualitative and quantitative analysis of glucomanno-oligosaccharides.