乳腺丝氨酸蛋白酶抑制剂在口腔鳞状细胞癌亚细胞中的表达及意义

Mammary serine proteinase inhibitor subcellular expression in oral squamous cell carcinoma and its clinical significance

目的检测乳腺丝氨酸蛋白酶抑制剂（mammaary serine proteinase inhibitor，Maspin）在口腔鳞状细胞癌亚细胞中的表达，观察其与口腔鳞状细胞癌患者临床病理特征之间的关系，以期为临床提供参考。方法应用免疫组织化学方法对45例口腔鳞状细胞癌的癌组织标本行Maspin蛋白含量的半定量测定，统计其亚细胞定位表达，并与各临床病理指标进行统计学分析。结果Maspin蛋白在口腔鳞状细胞癌胞核中表达的强阳性率为24％（11／45），弱阳性率为11％（5／45），阴性率为64％（29／45），细胞核表达与肿瘤大小（P=0．019）、淋巴结转移（P=0．011）及术后转移（P=0．017）呈负相关，与患者术后的生存时间呈正相关（P=0．030）；Maspin蛋白在口腔鳞状细胞癌胞质中表达的强阳性率为31％（14／45），弱阳性率为31％（14／45），阴性率为38％（17／45），细胞质表达与淋巴结转移（P=0．038）、术后转移（P=0．004）呈负相关，与患者术后的生存时间呈正相关（P=0．014）。结论Maspin蛋白在口腔鳞状细胞癌胞核或胞质中的表达对判断预后可能具有重要价值。

Objective To investigate the subcellular expression of mammary serine proteinase inhibitor （Maspin） in oral squamous cell carcinoma and its relationship to the clinicopathological features. Methods The Maspin protein subcellular expression was detected in 45 patients with oral squamous cell carcinoma by immunohistochemical staining. The relationship between the Maspin protein subcellular expression and the clinicopathologieal parameters was analyzed. Results The negative rate of nuclear maspin expression was 64% （29/45）, and the weakly positive rate was 11% （5/45）, and the strong positive rate was 24% （ 11/45 ）. Nuclear maspin expression was negatively correlated with T stage （ P = 0. 019）, lymph node metastasis （ P = 0. 038 ） and postoperative metastasis （ P = 0. 004 ）, but positively correlated with the patients＇ survival rate （P = 0. 014）. The negative rate of eytoplasmatic maspin expression was 38% （17/45）,and the weakly positive rate was 31% （14/45）, and the strong positive rate was 31% （14/45）. Cytoplasmatic maspin expression was negatively correlated with lymph node metastasis （P = 0. 038） and postoperative metastasis （P = 0. 004）, but positively correlated with the patients＇ survival rate （P = 0. 014）. Conclusions Maspin expression may be a significant marker in predicting prognosis of oral squamous cell carcinoma.