ROCK2蛋白在食管鳞状细胞癌中的表达及其临床意义

Relationship between ROCK2 expression and clinicopathologic characteristics of esophageal squamous cell carcinoma

目的研究Rho相关的蛋白激酶2（ROCK2）在食管鳞癌组织中的表达水平及其临床意义。方法收集1990至2001年间在中国医学科学院肿瘤医院接受手术治疗的原发食管癌病例，免疫组织化学方法检测了组织芯片中118例食管鳞癌组织及其配对的癌旁正常食管黏膜中ROCK2的表达水平。将病例按年龄、性别、肿瘤部位、吸烟与否、分化程度、T分期、淋巴结转移及TNM分期进行分组，比较不同组别中ROCK2表达水平的差异。结果ROCK2在118例食管鳞癌组织中的高表达率为45．76％（54／118）。在年龄〈61岁组，ROCK2高表达率为55．74％（34／61），高于年龄〉61岁组的35．09％（20／57），两者差异具有统计学意义（X2=5．062，P=0．024）。高分化病例组中ROCK2高表达率为58．70％（27／46），高于中、低分化病例组的37．50％（27／72），差异具有统计学意义（X2=5．080，P=0．024）。结论ROCK2在食管鳞癌组织中的表达升高与发病年龄及肿瘤的分化程度相关。

Objective To investigate the expression and relationship of Rho-associated protein kinase 2 （ROCK2） and clinical characteristics in esophageal squamous cell carcinoma （ESCC）. Methods Immunohistochemistry was performed to assay the expression of ROCK2 in tumor tissues and adjacent normal epithelium from 118 ESCC patients in tissue microarray. The relationship between ROCK2 level and clinicopathologic profiles such as age, gender, location, smoking, differentiation degree, T stage, lymph node metastasis and TNM stage were analyzed. Results The ROCK2 expression was up-regulated in 54 of 118 （45.76%） ESCC tissues. The up-regulated expression of ROCK2 was observed in 55.74% （34/61） ESCC tissues of patients under 61 years old. And it was significantly higher than that in 35.09% （20/57） of patients over 61 years old （ x2 = 5. 062, P = 0. 024 ）. In addition, the rate of up-regulation of ROCK2 was significantly higher in high-grade differentiation group （58. 70% , 27/46） than that in moderate-grade and low-grade differentiation group （ 37.50%, 27/72 ） （ X2 = 5. 080, P = 0. 024 ）. Conclusion The upregulated expression of ROCK2 is correlated with patient age and differentiation grade of ESCC.