结核分枝杆菌12种抗原在结核病血清学诊断中应用价值的研究

Serodiagnostic value of twelve antigens from M.tuberculosis for tuberculosis

目的研究结核分枝杆菌11种重组蛋白和1种脂阿拉伯甘露糖(LAM)的抗原性,评价其在结核病血清学诊断中的价值。方法将12种抗原包被于酶标板,通过酶联免疫吸附试验(ELISA)检测470例血清中抗结核抗体IgG水平,分析不同抗原组合对结核检出的灵敏度与特异度。结果单个抗原检测抗结核抗体的灵敏度介于13.3%(28/210)与40.5%(85/210)之间,特异度介于96.4%(185/192)与99.5%(191/192)之间,4种抗原联合检测的灵敏度和特异度分别达到65.2%(137/210)和92.2%(177/192)。结论多种抗原联合应用可提高结核病血清学诊断的灵敏度和特异度,有助于结核病的临床诊断。

Objective To evaluate the value of twelve antigens from M.tuberculosis on the serodiagnosis of tuberculosis（TB）,and to develop more effective diagnostic reagents for tuberculosis. Method 470 human sera were detected the antibodies against twelve M.tuberculosis antigens by ELISA,which sensitivities and specificities were evaluated. Results The sensitivities of antibody detected by single antigen were range from 13.3%（28/210） to 40.5%（85/210）,and the specificities were range from 96.4%（185/192） to 99.5%（191/192）.The sensitivity and specificity of antibodies detected by multiple antigens can reach 65.2%（137/210） and 92.2%（177/192）,respectively. Conclusion The multiple antigens can increase the sensitivity and specificity of anti-TB antibody detection,and it may be valuable in the diagnosis of TB.