摘要
当前的学习试图评估是否的目的由 Ox-LDL 的煽动性的 cytokines 与 ABCA1 的表示有关的巨噬细胞的正式就职小径。巨噬细胞是有处理与 Ox-LDL (30mg/L ) 跟随的 ABCA1 antisense oligonucleotides (100nmol/L ) 的 transfected 的在 THP1/PMA 以后的方法， ABCA1 的表情， ICAM-1 和 MCP-1 mRNA 和蛋白质被决定由即时荧光灯量的 RT-PCR，西方的污点或 ELISA。结果 Ox-LDL 从 THP1/PMA 巨噬细胞在 mRNA 和蛋白质层次导致了 ABCA1， ICAM-1，和 MCP-1 的表情。有 ABCA1 antisense oligonucleotides 的 Transfection 在 12 和 24 个小时以后在 3 和 6 个小时和蛋白质层次以后减少了 ABCA1 mRNA 层次。在由 ABCA1 antisense oligonucleotide 的 ABCA1 蛋白质表示的抑制减少了以后， Ox-LDL 导致的 ICAM-1 和 MCP-1 的表示也被减少。结论巨噬细胞的正式就职由 Ox-LDL 的煽动性的 cytokines 部分依赖于 ABCA1 的表示。我们的研究在巨噬细胞揭示 ABCA1 的新功能。
Objective The current study aimed to evaluate whether the induction of macrophage inflammatory cytokines by Ox-LDL is related to the expression of ABCA 1 pathway. Methods After THP 1/PMA macrophages were transfected with ABCA1 antisense oligonucleotides （100nmol/L） followed by treatment with Ox-LDL （30mg/L）, the expressions of ABCA1, ICAM-1 and MCP-1 mRNA and protein were determined by real-time fluorescent quantitative RT-PCR, Western blot or ELISA. Results Ox-LDL induced expressions of ABCA1, ICAM-1, and MCP-1 at both mRNA and protein levels from THPI/PMA macrophages. Transfection with ABCAI antisense oligonucleotides reduced ABCA1 mRNA levels after 3 and 6 hours and protein levels after 12 and 24 hours. The expression of ICAM-1 and MCP-1 induced by Ox-LDL was also decreased after inhibition of ABCA 1 protein expression by ABCA 1 antisense oligonucleotide decreased. Conclusion The induction of macrophage inflammatory cytokines by Ox-LDL is partially dependent on expression ofABCA1. Our studies disclose new functions of ABCA1 in macrophages Objective The current study aimed to evaluate whether the induction of macrophage inflammatory eytokines by Ox-LDL is related to the expression of ABCA 1 pathway. Methods After THP 1/PMA macrophages were transfected with ABCA1 antisense oligonucleotides （100nmol/L） followed by treatment with Ox-LDL （30mg/L）, the expressions of ABCA1, ICAM-1 and MCP-1 mRNA and protein were determined by real-time fluorescent quantitative RT-PCR, Western blot or ELISA. Results Ox-LDL induced expressions of ABCA1, ICAM-1, and MCP-1 at both mRNA and protein levels from THPI/PMA macrophages. Transfection with ABCAI antisense oligonucleotides reduced ABCA1 mRNA levels after 3 and 6 hours and protein levels after 12 and 24 hours. The expression of ICAM-1 and MCP-1 induced by Ox-LDL was also decreased after inhibition of ABCA 1 protein expression by ABCA 1 antisense oligonucleotide decreased. Conclusion The induction of macrophage inflammatory cytokines by Ox-LDL is partially dependent on expression ofABCA1. Our studies disclose new functions of ABCA1 in macrophages
