家蚕抗浓核病近等基因系及其RAPD分子标记

Breeding of Densonucleosis (DNV) Resistance Near isogenic Line and RAPD Molecular Markers

用抗浓核病的蚕品种东 3 4 (rr)与感病的品种菁松 (RR)杂交 ,再以菁松作轮回亲本 ,进行回交育种。由于家蚕抗浓核病基因表现隐性 ,因此 ,用测交添毒选择方法保留抗病基因 (r) ,通过回交 5代和自交一代的选择培育获得了抗浓核病的近等基因系菁东。对 2个品系及抗病亲本用 50个随机引物进行了RAPD分析。

Take silkworm variety of densonucleosis (DNV) resistance Dong34(rr) to cross with susceptible variety Jingsong(RR),then use the susceptible Jingsong as recurrent parent.Because silkworm densonucleosis resistance gene is recessive,it (r) can be reserved by test cross and virus inoculation.Through the selection breeding of 5 generations backcrosses and 1 generation self cross,the densonucleosis resistance Near isogenic line Jingdong has been gained.And RAPD analysis of two near isogenic lines and the DNV resistant parent were carried out with 50 random primers.