白术挥发油中极性和非极性成分的高效液相色谱法特征图谱研究

HPLC specific chromatograms of polarity and nonpolarity components in Rhizoma Atractylodis Macrocephalae volatile oil

目的建立挥发油中极性和非极性成分的高效液相色谱法(HPLC)特征图谱分析方法,分析比较4个产地挥发油中极性和非极性成分特征图谱中特征成分及其相对含量异同点。方法采用Hypersil ODS2(4.6mm×250mm,5μm)色谱柱:流动相乙腈(A)-水(B)60:40,梯度洗脱[0～30min,40%B→10%B;30～40min,10%B→10%B]:流速为1.0mL/min,柱温30℃;非极性成分特征图谱检测波长255nm,极性成分特征图谱检测波长205nm。结果挥发油中极性和非极性成分的HPLC特征图谱分析方法考察结果良好。4个产地挥发油中极性和非极性成分特征图谱与共有模式之间相似度≥0.996。4个产地挥发油中极性和非极性成分特征图谱及共有模式之间特征峰的相对保留时间相对标准偏差(RSD)≤0.89%,相对峰面积RSD为21%～79%。4个产地挥发油中极性和非极性成分特征图谱中特征峰的峰面积占总峰面积为97.65%～98.30%。浙江、安徽、湖南产地挥发油中极性和非极性成分特征图谱及共有模式之间特征峰的相对保留时间RSD≤2.19%,相对峰面积RSD≤2.39%;与河北的进行比较,相对保留时间RSD≤1.68%,相对峰面积RSD5.6%～68%。结论本研究建立挥发油中极性和非极性成分的HPLC特征图谱分析方法简单、稳定可靠。4个产地挥发油中极性和非极性成分的特征图谱的整体相似度较高,特征成分的种类相近,但其含量差异明显。浙江、安徽、湖南产地白术挥发油中极性和非极性成分的特征图谱中的特征成分的种类及其相对含量相近;河北的与之相比,特征峰的种类相近,但含量有较大差异。

Objective To establish the analytical method for study of HPLC specific chromatograms of polarity and nonpolarity components in Rhizoma Atractylodis Macrocephalae volatile oil,and to identify and compare the characteristic polarity and nonpolarity components and their levels in specific chromatograms of volatile oil from four different origins.Methods This study was conducted with Hypersil ODS2 （4.6 nm×250 mm,5μm） column;acetonitrile （A）-water （B） 60：40,gradient elution [0～30min,40%B→10%B;30～40min,10%B→10%B];flow rate 1.0 mL/min,column temperature 30℃.The wavelength was 255 nm for detection of nonpolarity components,and 205 nm for detection of polarity components.Results The analytical method for HPLC specific chromatograms of polarity and nonpolarity components in volatile oil worked favorably.Among the polarity and nonpolarity components from volatile oils for four different origins,the similarity between specific chromatograms and common modules were ≥0.996,the relative retention time of characteristic peak RSD was ≤0.89% and relative peak area RSD ranged from 21% to 79%.In the specific chromatograms,the characteristic peak area accounted for 97.65%～98.30% of the total peak area.The relative retention time of characteristic peak RSD was ≤2.19% and relative peak area RSD ≤2.39% between characteristic chromatograms and common modules in polarity and nonpolarity components of volatile oil from Zhejiang,Anhui and Hu＇nan.The relative retention time RSD was ≤1.68% and relative peak area RSD ranged from 5.6% to 68% as compared with products from Hebei.Conclusion The established analytical methods of HPLC characteristic chromatograms of polarity and nonpolarity components in volatile oil were simple,stable and reliable.The specific chromatograms of polarity and nonpolarity components in volatile oil from four different origins had a relatively high overall similarity.Although the characteristic components were similar on specific chromatogram,the contents of these components showed significant difference.The specific chromatograms of polarity and nonpolarity components in volatile oil from Zhejiang,Anhui and Hu＇nan had similar characteristic components and relative contents.In contrast,products from Hebei showed similar components but significantly different contents.