摘要
目的研究新城疫病毒(NDV)对卵巢癌细胞株(SKOV-3)的杀伤作用。方法用不同浓度新城疫病毒(NDV-D90)与卵巢癌细胞株(SKOV-3)共培养;在不同时间(6、12、24、48h)观察SKOV-3细胞形态的改变。结果 NDV病毒作用的SKOV-3细胞表现为细胞膜皱缩、细胞核染色质浓缩、细胞溶解和细胞凋亡;NDV可对SKOV-3胞产生抑制作用,抑制效果与浓度及共同孵育时间有关,差异有统计学意义(P<0.01)。结论 NDV对体外培养SKOV-3细胞增殖有明显抑制作用,并可诱导该细胞凋亡。
Objective To investigate the effect of newcastle disease virus-infected （ NDV ） on the SKOV-3. Methods NDV-D90 in different concentration was cultured with SKOV-3 ; The SKOV-3 cells were monitored for cell morphological changes under micro scope within 6hours, 12hours,24hours and 48hours respectively. Results The SKOV3 cells infected by NDV become shrinked, dissolved. Cell concentration and apoptotic bodies were observed as well. NDV could effectively inhibit SKOV-3 cell growth, which depends on virus concentration and inoculation time of NDV and the difference of cell growth inhibitory rate among ceils was significant （P 〈 0.01 ）. Conclusion NDV can inhibit proliferation and induces cell apoptosis in SKOV-3 cells in vitro.
出处
《中国现代医生》
2011年第23期25-26,45,共3页
China Modern Doctor
