前列腺干细胞抗原在膀胱尿路上皮癌组织中的表达及其临床意义

Expression of PSCA protein in human bladder urothelial cancer and its clinical significance

目的探讨前列腺干细胞抗原(PSCA)在膀胱尿路上皮癌组织中的表达情况,并分析其与膀胱癌临床病理特征及复发的关系。方法选择105例膀胱尿路上皮癌标本,20例癌旁正常膀胱组织标本。癌标本中非肌层浸润性癌74例,肌层浸润性癌31例;G126例、G254例、G325例;单发肿瘤63例,多发肿瘤42例;非肌层浸润性癌中未复发31例,复发43例。采用SP免疫组织化学方法检测膀胱癌和癌旁正常组织中PSCA蛋白的表达,并结合临床病理资料进行分析。结果正常膀胱组织中无PSCA蛋白表达,癌组织中随病理分级和分期增加,PSCA相对表达量逐渐增高。G1、G2、G3膀胱癌PSCA蛋白表达阳性率分别为23.1%、46.3%,76.0%,差异均有统计学意义(P<0.001)。非肌层浸润性肿瘤和肌层浸润性肿瘤中PSCA蛋白表达阳性率为47.3%和71.0%,差异有统计学意义(P=0.004)。随访3～54个月,非肌层浸润性膀胱癌中,复发组与未复发组中PSCA蛋白高表达阳性率为65.1%和32.3%,差异有统计学意义(P<0.001)。结论 PSCA蛋白高表达与膀胱尿路上皮癌的恶性程度及预后相关,检测PSCA有助于膀胱癌的诊断及评估预后。

Objective To explore the expression of prostate stem cell antigen （PSCA） in bladder urothelial cancer and analyze its clinical significance and relationship with tumor recurrence. Methods The expressions of PSCA protein in 105 specimens of bladder cancer and 20 specimens of normal bladder tissues were detected by immunohistochemical staining and its clinical significance was then analyzed. Resuits The expression of PSCA protein was higher in bladder cancer than that in the adjacent normal tissues （P〈0.05）. PSCA protein expression rates were 23.1%, 46.3%, and 76.0% in G1, G2,and G3 tumors,respectively （P〈O.001）. Expression of PSCA protein was higher in muscle invasive bladder cancers than in nonmuscle invasive bladder cancer （71.0% versus 47.3%, P=0.004）. In patients with non-muscle invasive bladder cancer, PSCA protein expression was significantly higher in patients with recurrence than in those without recurrence （65.1% versus 32.3%, P〈0.001）. Conclusion PSCA may play a role in the development of bladder urothelial cancer. The determination of PSCA protein expression is potentially valuable in diagnosis and prognostic evaluation for bladder urothelial cancers.