视黄醇结合蛋白4、核因子κB的表达与糖尿病大鼠动脉粥样硬化的相关性

The relationship of expressions of RBP4 and NF-κB with atherosclerosis in diabetic rats

目的探讨视黄醇结合蛋白4(RBP4)、主动脉壁核因子κB(NF-κB)与糖尿病大血管病变的关系。方法 Wistar大鼠分为:对照组(NC组)、单纯糖尿病组((DM组)、糖尿病合并动脉粥样硬化组(DM+AS组)。测大鼠主动脉NF-κB活性、血清和附睾RBP4水平、FBG、FIns、TG、HDL-C、LDL-C、尾动脉SBP,计算血浆致动脉粥样硬化指数(AIP)及胰岛素抵抗指数(HOM-IR)。结果 DM组和DM+AS组的RBP4、NF-κB、TG、LDL-C、FBG、FIns、SBP、AIP、HOMA-IR高于NC组;DM+AS组上述指标高于DM组;RBP4与TG、LDL-C、HOMA-IR、AIP、NF-κB、SBP、体脂比成正相关,与HDL-C成负相关。RBP4、TG是糖尿病大血管病变发生的独立危险因素。结论 NF-κB与RBP4与糖尿病大血管病变发生相关,RBP4可能通过胰岛素抵抗、炎症机制及脂代谢紊乱参与糖尿病大血管病变的发生。

Objective To investigate the correlation of expression of retionl binding protein-4 and NF-κB with atherosclerosis in diabetic rats. Methods 55 male Wista rats were randomly divided into three groups： normal control （NC group）, diabetes rats （DM group）, diabetes rats with atherosclerosis （DM＋AS group）. The activity of nuclear factor-kappa B in aorta, the RBP levels of serum and adipose tissue, serum glucose, insulin, HDL-C, LDL-C, TG, and SBP were measured. The ratio of visceral adipose tissue over body weight, atherogenie index of plasma （AIP） and insulin resistance index（HOMA-IR） were calculated. Results The values of TG, LDL-C, FBG, Fins, RBP4, NF-κB, HOMA-IR, and AIP were significantly higher in group of DM and DM＋ AS than in NC group, while HDL-C was decreased. Compared with group DM, above-mentioned indexes were increased in group DM ＋ AS. Correlation analysis suggested that there was a positive correlation of RBP4 with TG, LDL-C, HOMA-IR, AIP, NF- κB, and a negative correlation between RBP4 and HDL-C. RBP4 and TG were independent risk factors for the progression of maerovascular complication of diabetes mellitus. Conclusion RBP4 and NF-κB are related with macrovascular diseases. RBP4 is an independent risk factor for the progression of macrovascular complication of diabetes mellitus through induction of insulin resistance, inflammation and disorder of fat metabolism.