HPLC测定热毒平颗粒中木犀草苷的含量

Determination of Luteoloside in Reduping Granules by HPLC

目的:研究热毒平颗粒中木犀草苷的含量测定方法。方法:采用HPLC梯度洗脱法。色谱柱为Thermo HypeisilGOLD(4.6 mm×250 mm,5μm),以乙腈-0.5%冰醋酸水溶液为流动相,流速1 mL.min-1,柱温30℃;检测波长350 nm。结果:木犀草苷在0.034 7～0.694μg呈良好的线性关系(r=0.999 9),平均回收率为99.3%,RSD为0.5%。结论:所建立的方法能准确可靠地进行定量检测,可有效地控制制剂质量。

Objective： To study the method of determining luteoloside in Reduping Granules.Method： The content of luteoloside in Reduping Granules was determined on an Thermo Hypeisil GOLD column（4.6 mm×250 mm,5 μm） eluted with acetonitrile and water containing 0.5% glacial acetic acid as mobile phases in gradient mode.The flow rate was kept at 1.0 mL·min-1,and the column temperature was set at 30 ℃.The UV detector wavelengths were at 350 nm.Result： Luteoloside was linear in the range of 0.034 7-0.694 μg（r=0.999 99）.The average recovery was 99.3% and RSD was 0.5%.Conclusion： The established method is accurate and reliable,can be used to control the quality of this preparation effectively.