高效液相色谱法测定接骨挫伤胶囊中士的宁含量

Determination of Strychnine in Jiegu Cuoshang Capsule by HPLC

目的:建立接骨挫伤胶囊中士的宁的含量测定方法。方法:DIKMA C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-水-冰醋酸-三乙胺(76∶230∶2.4∶0.3),检测波长254 nm,流速1.20 mL.min-1,柱温30℃。结果:士的宁在0.06～1.20μg进样量与峰面积呈良好的线性关系,平均加样回收率为98.2%,RSD 2.2%。结论:该方法简单,重复性好,可用于接骨挫伤胶囊的质量控制。

Objective：To establish an HPLC method for the content determination of strychnine in Jiegu Cuoshang capsule. Method：HPLC was performed on DIKMA C18 column（4.6 mm×250 mm, 5 μm）,the mobile phase consisted of methanol-water-glacial acetic acid-triethylamine （76∶230∶2.4∶0.3）and detecting at 254 nm.The flow rate was 1.20 mL·min-1,and the temperature was 30 ℃. Result：The linear range of strychnine was 0.06-1.20 μg（r=0.999 8）. The average recovery was 98.2%, and RSD was 2.2%.Conclusion：The method is simple, feasible, and reproducible, and it can be used for the quality control of Jiegu Cuoshang Capsule.