期刊文献+

补肾活血方对免疫性卵巢早衰小鼠的影响 被引量:13

Effect of Reinforcing Kidney and Activating Blood Prescription on Autoimmune Premature Ovarian Failure in Mice
原文传递
导出
摘要 目的:探讨补肾活血方对免疫性卵巢早衰(POF)小鼠的治疗作用。方法:以重组猪卵透明带(pZP4)蛋白两后脚掌和皮下多点注射免疫雌性BALB/c小鼠建立POF动物模型。小鼠随机分为正常组、模型组、雌二醇组和补肾活血方高、中、低剂量组(按生药量计分别为15.0,7.5,3.75 g.kg-1),分析ig 20 d后阴道脱落细胞涂片、外周血雌二醇(E2)浓度、血清抗透明带(pZP4)抗体变化及卵巢组织炎形态学观察。结果:补肾活血方高剂量组小鼠阴道涂片显示动情周期基本规则为6~7 d;高剂量组可明显升高小鼠血清E2浓度(P<0.01);高剂量组小鼠的血清抗pZP4抗体滴度显著下降(P<0.05);高剂量组小鼠初级卵泡和次级卵泡的数量显著增加(P<0.05)。结论:补肾活血方能改善卵巢的生殖内分泌功能和卵巢内各期卵泡和黄体的发育状况,并可在一定程度上抑制自身免疫早衰作用。 Objective:To investigate the therapeutical effect of a Chinese prescription with the function of reinforcing kidney and activating blood on immune premature ovarian failure(POF) in mice.Method:Each female BALB/c mouse was received multipoint immunization of porcine ZP4(pZP4) in two hind footpads and intra-cutaneously to made the POF model.The mice were randomly divided into three Chinese medicine groups,contorl group,model group and estradiol(E2) group.After administrating Chinese medicine in three different doses(15.0,7.5,3.75 g·kg-1),0.5% CMC-Na and E2 were given respectively for 20 days,vaginal smearses were visualized microscopically.E2 and pZP4 antibody levels were monitored.The morphology of ovarian tissue inflammation were studied.Result:Compared to the control group,the sex cycles of the high dose group was between 6 and 7 days without apparent change.Compared to the model group,the high dose group developed significantly higher serum E2 level(P0.01) and lower serum antiboides to pZP4(P0.05),and moreover,the number of primary follicles and secondary follicles were increased significantly(P0.05).Conclusion:The Chinese prescription can improve the ovarian reproductive endocrine function and regulate the developmental capacity of ovarian follicle and corpus luteum in the four cycles.Otherwise,the Chinese medicine might inhibit the autoimmune presenility POF to some extent.
出处 《中国实验方剂学杂志》 CAS 北大核心 2011年第16期137-140,共4页 Chinese Journal of Experimental Traditional Medical Formulae
基金 国家科技部(重大新药创制2009ZX09313-032)
关键词 卵巢早衰 雌二醇 透明带抗体 生殖内分泌 premature ovarian failure estradiol zona pellucida antibodies reproductive endocrine
  • 相关文献

参考文献8

二级参考文献52

  • 1蔡华普,徐晶,孟庆玲.卵巢功能早衰中医药治疗近况[J].山东中医杂志,2004,23(9):574-575. 被引量:12
  • 2朱玲,罗颂平,欧汝强.卵巢早衰的免疫学研究进展[J].生殖医学杂志,2005,14(2):120-124. 被引量:9
  • 3李大金,李超荆,朱影.滋阴降火中药治疗免疫性不孕症[J].中国中西医结合杂志,1995,15(1):3-5. 被引量:46
  • 4严敬明 李超荆 等.猪卵透明带的分离和提纯[J].上海医科大学学报,1987,14:358-366.
  • 5李大金 李超荆 等.BA-ELISA检测透明带抗体[J].上海免疫学杂志,1988,8:296-298.
  • 6李超荆.虚证研究[M].上海:上海科学技术出版社,1991.71-76.
  • 7李大金 李超荆.肾主生殖理论体系的科学内涵.中药药理与临床研究进展,第4册[M].北京:军事医学科学出版社,1996.356-364.
  • 8李大金,中药药理与临床研究进展.4,1996年,356页
  • 9Tung K S K,Horm Metab Res,1995年,27卷,539页
  • 10Lou Y H,J Immunol,1995年,7卷,3667页

共引文献202

同被引文献169

引证文献13

二级引证文献109

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部