牛膝饮片及牛膝多糖对小鼠免疫抑制调节作用的研究

Effects of Achyranthis Bidentatae Radix and Achyranthes Bidentata Polysaccharides on Enhancing Immune Function

目的:研究牛膝饮片及其有效成分牛膝多糖对免疫功能的影响,探讨二者作用的一致性。方法:将昆明种小鼠分为空白对照组、模型组、牛膝饮片组、牛膝多糖组及香菇菌多糖阳性对照组,ip环磷酰胺3 d造成小鼠免疫抑制模型,同时分别ig生理盐水及相应药物7 d后测定牛膝饮片、牛膝多糖对小鼠胸腺、脾脏质量、吞噬百分率、吞噬指数、溶血素、溶血斑以及淋巴细胞转化率的影响。结果:牛膝饮片、牛膝多糖均能提高环磷酰胺造成免疫抑制模型小鼠胸腺、脾脏质量、吞噬百分率、吞噬指数,促进溶血素、溶血斑形成,提高淋巴细胞转化率;与模型组相比,两者均能提高免疫抑制小鼠各免疫指数,且差异显著(P<0.01或P<0.05);在本实验用药剂量下(牛膝饮片20 g.kg-1,牛膝多糖0.5 g.kg-1)2者作用强度相当。结论:牛膝多糖是牛膝中增强免疫的主要有效成分。

Objective： To study the effect of Achyranthis Bidentatae Radix and achyranthis bidentatae polysaccharides（ABPS） on the mice＇s immune function,and investigate the consistency of both effects.Method： Kunming mice were divided into 5 groups： blank control group,immunosuppression model group,decoction group of Achyranthis Bidentatae Radix,ABPS group and mushroom polysaccharides group.The immunosuppression model of mouse was established with injecting cyclophosphamide abdominally.The effects of the decoction of Achyranthes Bidentatae Radix and ABPS on thymus weight,spleen weight,percentage of phagocytosis,phagocytic index,hemolysin,hemolysis of plaque and lymphocyte transformation rate were compared.Result： The decoction of Achyranthes Bidentatae Radix and ABPS both could improve model mice spleen weight,phagocytic percentage and phagocytic index,promote the formation of hemolysin and hemolytic plaque,and increase the rate of lymphocyte transformation.Compared with model group,both of them could improve immune indexes of the immunosuppressed mouse,and there was significant difference（P0.01 or P0.05）.With the dosage in this study,the effect of the decoction of herbal pieces at 20 g·kg-1 was equal to that of ABPS at 0.5 g·kg-1 for improving immune function.Conclusion： ABPS is the main effective composition of Achyranthes Bidentata Radix for reinforcing immune function.