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大黄素对小鼠缺血再灌注肠黏膜肥大细胞活性的影响 被引量:6

Effect of Emodin on Intestinalmucosa Mast Cells in Mice with Intestine Ischemia-Reperfusion
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摘要 目的:研究大黄素对小鼠肠缺血再灌注肠黏膜肥大细胞活性的影响。方法:28只昆明种小鼠随机均分为4组,假手术组(A组)、模型组(B组)、模型+大黄素60 mg.kg-1组(D1组)及模型+大黄素120 mg.kg-1组(D2组)。采用肠系膜上动脉夹闭法建立小肠缺血再灌注模型,观察肠黏膜病理结构变化、肠黏膜肥大细胞超微结构变化及类胰蛋白酶表达、比较计算肥大细胞数量,测定小肠组织组胺、肿瘤坏死因子-α(TNF-α)浓度。结果:与假手术组比较,模型组Chiu’s评分、肥大细胞数量、组胺及TNF-α浓度显著增加(P<0.05或P<0.01)。与模型组比较,大黄素60,120 mg.kg-1组的肥大细胞数量明显减少(P<0.05),大黄素120 mg.kg-1组的小肠组织TNF-α浓度明显降低(P<0.05)。假手术组肥大细胞超微结构正常,模型组肥大细胞颗粒包膜相互融合形成细胞内空泡等脱颗粒现象,大黄素60,120 mg.kg-1组肥大细胞形成空泡较少。结论:大黄素能减少小鼠小肠黏膜结构破坏,抑制小肠肥大细胞活化及脱颗粒,从而起到防治肠缺血再灌注损伤的作用。 Objective: To investigate the effect of emodin on intestinalmucosa mast cells(IMMC) in mice with intestine ischemia-reperfusion(I/R).Method: Twenty-eight mice were randomly divided into four groups(n=7 each): group A(sham operation group),group B(superior mesenteric artery was clamped for 30 minutes followed by reperfusion),group D1 and D2(in which emodin 60 or 120 mg·kg-1 was given via gastric tube every day for 3 days before I/R).Intestinalmucosa pathology structure,the ultramicrostructure of IMMC were observed under electron microscope,and tryptase expression was compared for IMMC counting,histamine and tumor necrosis factor(TNF-α) were determined.Result: Compared with that of group A,Chiu's score and IMMC number,histamine,TNF-α in group B were increased significantly(P0.05 or P0.01).Compared with that of group B,IMMC number of group D1,D2 and TNF-α of group D2 were decreased markedly(P0.05).IMMC ultrastructure in group A was normal,and there was some degranulation phenomenon in group B,such as mast cell envelope coalition and intracellular vacuoles.There was more less intracellular vacuoles in group D1 and D2.Conclusion: Emodin can decrease intestinalmucosa disorganization in mice by inhibiting IMMC activation and degranulation.
出处 《中国实验方剂学杂志》 CAS 北大核心 2011年第16期167-170,共4页 Chinese Journal of Experimental Traditional Medical Formulae
基金 广东省中医药管理局立项资助项目(2008264)
关键词 大黄素 肠黏膜肥大细胞 缺血再灌注 小鼠 emodin intestinalmucosa mast cells ischemia-reperfusion mice
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