大黄素对小鼠缺血再灌注肠黏膜肥大细胞活性的影响

Effect of Emodin on Intestinalmucosa Mast Cells in Mice with Intestine Ischemia-Reperfusion

目的:研究大黄素对小鼠肠缺血再灌注肠黏膜肥大细胞活性的影响。方法:28只昆明种小鼠随机均分为4组,假手术组(A组)、模型组(B组)、模型+大黄素60 mg.kg-1组(D1组)及模型+大黄素120 mg.kg-1组(D2组)。采用肠系膜上动脉夹闭法建立小肠缺血再灌注模型,观察肠黏膜病理结构变化、肠黏膜肥大细胞超微结构变化及类胰蛋白酶表达、比较计算肥大细胞数量,测定小肠组织组胺、肿瘤坏死因子-α(TNF-α)浓度。结果:与假手术组比较,模型组Chiu’s评分、肥大细胞数量、组胺及TNF-α浓度显著增加(P<0.05或P<0.01)。与模型组比较,大黄素60,120 mg.kg-1组的肥大细胞数量明显减少(P<0.05),大黄素120 mg.kg-1组的小肠组织TNF-α浓度明显降低(P<0.05)。假手术组肥大细胞超微结构正常,模型组肥大细胞颗粒包膜相互融合形成细胞内空泡等脱颗粒现象,大黄素60,120 mg.kg-1组肥大细胞形成空泡较少。结论:大黄素能减少小鼠小肠黏膜结构破坏,抑制小肠肥大细胞活化及脱颗粒,从而起到防治肠缺血再灌注损伤的作用。

Objective： To investigate the effect of emodin on intestinalmucosa mast cells（IMMC） in mice with intestine ischemia-reperfusion（I/R）.Method： Twenty-eight mice were randomly divided into four groups（n=7 each）： group A（sham operation group）,group B（superior mesenteric artery was clamped for 30 minutes followed by reperfusion）,group D1 and D2（in which emodin 60 or 120 mg·kg-1 was given via gastric tube every day for 3 days before I/R）.Intestinalmucosa pathology structure,the ultramicrostructure of IMMC were observed under electron microscope,and tryptase expression was compared for IMMC counting,histamine and tumor necrosis factor（TNF-α） were determined.Result： Compared with that of group A,Chiu＇s score and IMMC number,histamine,TNF-α in group B were increased significantly（P0.05 or P0.01）.Compared with that of group B,IMMC number of group D1,D2 and TNF-α of group D2 were decreased markedly（P0.05）.IMMC ultrastructure in group A was normal,and there was some degranulation phenomenon in group B,such as mast cell envelope coalition and intracellular vacuoles.There was more less intracellular vacuoles in group D1 and D2.Conclusion： Emodin can decrease intestinalmucosa disorganization in mice by inhibiting IMMC activation and degranulation.