圣愈汤对骨髓抑制小鼠骨髓细胞周期和凋亡的影响

Effects of Sheng-Yu Decoction on Bone Marrow,Cell Cycle and Apoptosis of Myelosuppressed Mice

目的:观察圣愈汤对骨髓抑制小鼠外周血、骨髓细胞周期和凋亡的影响,并探讨其造血调控的可能机制。方法:将60只BALB/c小鼠随机分为空白组、模型组、阳性药组、圣愈汤32,16,8 g.kg-1.d-1 3个剂量组,每组各10只,除空白组外,其他5组用60Coγ照射和ip注射环磷酰胺、氯霉素制备骨髓抑制小鼠模型。造模完成24 h后,空白组和模型组小鼠ig生理盐水,圣愈汤三剂量组分别按含生药32,16,8 g.kg-1.d-1ig给药,给药体积20 mL.kg-1.d-1;阳性组ip rhG-CSF 125μg.kg-1.d-1,各组均连续给药7 d。用全自动血细胞分析仪、白细胞计数法、流式细胞术(FCM)分别检测圣愈汤对骨髓抑制小鼠外周血、骨髓有核细胞数、骨髓细胞周期和凋亡率的影响。结果:圣愈汤32,16 g.kg-1.d-1均能明显升高外周血白细胞(WBC)、红细胞(RBC)、血红蛋白(Hb)、血小板(PLT)和骨髓有核细胞(BMC)数(P<0.05或P<0.01)。模型组、圣愈汤32,16 g.kg-1.d-1组G0/G1期细胞比例分别为(70.38±1.38)%,(62.43±2.03)%,(63.85±2.90)%;S期比例分别为(22.52±1.27)%,(26.35±2.52)%,(24.80±1.48)%;G2/M期细胞比例(7.43±1.04)%,(11.37±2.18)%,(10.95±2.35)%;PI分别为(29.85±1.06)%,(37.66±2.01)%,(35.88±3.13)%;凋亡率分别为(26.88±3.58)%,(19.15±4.30)%,(21.65±4.34)%。圣愈汤32,16g.kg-1.d-1组与模型组比,上述指标有显著差异(P<0.05或P<0.01)。结论:圣愈汤能改善骨髓抑制小鼠的造血机能,其机制之一可能是通过促进骨髓造血细胞进入细胞增殖周期和抑制其凋亡。

Objective： To observe the effect of Sheng-Yu decoction on peripheral blood cells,bone marrow cell cycle and apoptosis in myelosuppressed mice and to explore its possible mechanism in hematopoietic regulation.Method： The 60 BALB / c mice were randomly divided into blank group,model group,positive medicine group,Sheng-Yu decoction,three dose groups（32,16,8 g·kg-1·d-1）,with 10 mice in each group.Except the blank group.The other 5 groups were established the model of myelosuppression by irradiated 60Coγ and injection of cyclophosphamide and chloramphenicol.After 24 h of the completion of modeling,blank group and model group were ig normal saline,three doses of Sheng-Yu decoction groups were respectively ig corresponding crude drug 32,16,8 g· kg-1·d-1,with drug volume 20 mL·kg-1·d-1;and the positive control group were injected with rhG-CSF 125 ug· kg-1·d-1.Each group was medicated for 7 days.The effect of Sheng-Yu decoction on peripheral blood cells,bone marrow nucleated cells（BMNCs） and bone marrow cell cycle and apoptosis in myelosuppressed mice was detected by automatic blood cell analyzer,white blood cell counting and flow cytometry（FCM） respectively.Result： Peripheral white blood cells,red blood cells,hemoglobin,blood platelet and BMNCs were increased obviously by Sheng-Yu Granul,groups（P0.05 or P0.01）.In model group,Sheng-Yu decoction（32,16 g·kg-1·d-1） dose groups,the proportion of G0/G1 phase cells were（70.38±1.38）%,（62.43±2.03）%,（63.85±2.90）%;S phase fraction was（22.52±1.27）%,（26.35±2.52）%,（24.80±1.48）%;G2 / M phase cells（7.43±1.04）%,（11.37±2.18）%,（10.95±2.35）%;PI were（29.85±1.06）%,（37.66±2.01）%,（35.88±3.13）%;apoptotic rates were（26.88±3.58）%,（19.15±4.30）%,（21.65±4.34）% respectively.Compared with the model group,the above parameters of Sheng-Yu Granul,groups were significantly different（P0.05 or P0.01）.Conclusion： Sheng-Yu decoction could promote erythroid hematopoiesis by accelerating bone marrow cells of proliferation and diferentiation,inhibiting the apoptosis of hemopoietie cells.