Radicamine B体外抑制小肠葡萄糖的吸收作用

Radicamine B Inhibits Glucose Absorption in Rat Intestines in vitro

目的:探讨radicamine B对大鼠体外小肠葡萄糖吸收的影响。方法:采用α-葡萄糖苷酶抑制实验及酶抑制动力学实验和离体大鼠小肠葡萄糖吸收模型来研究radicamine B的抑制作用。结果:radicamine B对α-葡萄糖苷酶和大鼠体外小肠葡萄糖的吸收均呈剂量依赖性抑制作用,其IC50分别为2.19,0.094 g.L--1,与拜糖平相比较无显著性差异。而且radicamineB对α-葡萄糖苷酶活性属于竞争性抑制,Ki=6.3×10-7 mol.L-1。结论:radicamine B能显著抑制小肠葡萄糖的吸收,有望成为一种治疗糖尿病的药物。

Objective： To investigate the inhibitory effect of radicamine B on glucose absorption in rat intestines in vitro.Method： The inhibitory effect of radicamine B was evaluated by α-glucosidase inhibitory test,inhibitory kinetics assay and assay of glucose absorption in rat intestines in vitro.Result： Radicamine B showed an inhibitory effect on both α-glucosidase and glucose＇s absorption in the small intestine in a dose-dependent manner and the IC50 value were 2.19 g·L-1 and 0.094 g·L-1,respectively.No significant difference was found between acarbose and radicamine B in the inhibitory effect on intestinal glucose absorption.In addition,the inhibitory pattern of radicamine B was competitive enzyme inhibition with its Ki of 6.3×10-7 mol·L-1.Conclusion： Radicamine B significantly inhibites glucose absorption in the small intestine.It indecates that radicamine B might be a novel therapeutic drug for diabetes.