补肾活血方药对CAA患者骨髓单个核细胞C-met SF基因表达的影响

Effect of Bushen Huoxue Decoction on Expression of Bone Marrow Mononuclear Cell C-met SF Gene in Patients with Chronic Aplastic Anemia

目的:探讨补肾活血方药对慢性再生障碍性贫血(CAA)患者骨髓造血细胞C-met SF基因表达的影响。方法:60例CAA病人随机分为治疗组和对照组,治疗组给予补肾活血中药汤剂,每日1剂,分2次口服;对照组给予再障生血片,5片,每日3次,口服,疗程为3个月,采用逆转录-聚合酶链反应(RT-PCR)法检测CAA患者治疗前后骨髓单个核细胞C-met SFmRNA基因含量的变化。结果:CAA患者骨髓单个核细胞C-met SF mRNA表达水平高于正常对照组,治疗后C-met SF mRNA表达水平有不同程度下降,并且补肾活血方药对C-met SF表达水平的下调程度优于再障生血片组(P<0.05)。结论:CAA患者骨髓C-met SF的基因表达存在异常;补肾活血方药可能通过调节骨髓C-met SF基因的表达而改善CAA的骨髓造血功能,促进骨髓造血功能恢复。

Objective： To investigate the clinical effect of Bushen Huoxue Decoction on chronic aplastic anemia（CAA） patients,and observe the effect on expression level of myelo-cytokine C-met SF.Method： Sixty CAA cases were randomly divided into treatment group and control group.Treatment group： the patients were given Bushen Huoxue Decoction,and one dose divided into 2 times daily.Control group： the patients were given Zaizhang Shengxue tablet and each took 5 pills 3 times daily.The course of treatment was three months.This experiment employed reverse transcription-polymerase chain reaction（RT-PCR） method to exam the change of expression level of bone marrow mononuclear cell（BMMC） C-met SF receptor mRNA.Result：The expression level of BMMC C-met SF receptor mRNA in CAA patients was higher than that in normal control group.And in both treatment groups the expression levels were descended in different degree.Moreover,the descent in Bushen Huoxue Decoction group was superior to that in Zaizhang Shengxue tablet group.Conclusion： There was abnormal expression of bone marrow SCF receptor C-met SF in CAA patients;Bushen Huoxue Decoction can improve the hematopoietic function of bone marrow to promote restoration by regulating the bone marrow C-met SF mRNA.