肠淋巴再灌注对SMAO休克大鼠多器官ICAM-1、RAGE的作用

Role of Mesenteric Lymph Reperfusion on ICAM-1,RAGE of Multiple Organs in SMAO Shock Rats

目的:观察肠淋巴再灌注(MLR)对肠系膜上动脉闭塞性(SMAO)休克大鼠肺、心肌、肾、肝组织细胞间黏附分子-1(ICAM-1)、晚期糖基化终末产物受体(RAGE)含量的影响,探讨MLR加剧SMAO休克大鼠器官损伤的作用机制。方法:Wistar大鼠24只,随机均分为假手术组(Sham组)、MLR组、SMAO组和SMAO+MLR组,后三组大鼠分别夹闭肠系膜淋巴管和/或肠系膜动脉1h、再灌注2h。Sham组不夹闭。各组于相应时间点留取固定位置的肺、心肌、肾、肝组织,制备组织匀浆;应用酶联免疫方法检测各组织匀浆ICAM-1、RAGE含量。结果:Sham组和MLR组各指标无统计学差异;SMAO组和SMAO+MLR组肺、肾、心肌、肝组织ICAM-1、RAGE的含量均显著高于MLR组和Sham组(P均<0.01);SMAO+MLR组肺、肾、心肌、肝组织ICAM-1与RAGE含量显著高于SMAO组(P均<0.01)。结论:MLR加剧SMAO休克大鼠多器官损伤的作用机制与各器官组织ICAM-1、RAGE水平升高有关。

Objective: To observe the effects of mesenteric lymph reperfusion(MLR) on the contents of intercellular adhesion molecule-1(ICAM-1) and receptor of advanced glycation end-products(RAGE) of lung,myocardium,kidney,liver in superior mesenteric artery occlusion(SMAO) shock rats,and investigate the mechanism of MLR aggravating organ injury in SMAO shock.Method: Twenty four Wistar rats were radomly divided into sham group,MLR group,SMAO group,SMAO+MLR group,after performing 1h occlusion of mesenteric lymphatic duct(MLD) and/or superior mesenteric artery(SMA) followed by 2h of reperfusion in lasted three groups,and corresponding time point in sham group,the lung,myocardium,liver,kidney in fixed position were prepared for making homogenate respectively.And the contents of ICAM-1 and RAGE in homogenates were determined with enzyme-linked immunosorbent assay method.Results: The indices were no statistics difference between sham group and MLR group.The ICAM-1 and RAGE contents of the pulmonary,myocardial,renal,hepatic homogenates in SMAO and SMAO+MLR groups were significant higher than that of sham and MLR groups,and these indices in SMAO+MLR were increased significantly than those in SMAO group.Conclusion: The mechanism of MLR aggravates multiple organs injury in SMAO shock may be related to increase the ICAM-1 and RAGE levels in multiple organs.