3号染色体两单核苷酸多态性与中国前列腺癌风险的相关性研究

Two single nucleotide polymorphisms on chromosome 3 and the risk of prostate cancer in Chinese men

目的:探讨3号染色体常见单核苷酸多态性(SNP)与中国前列腺癌(PCa)发病风险的关系,并探讨其与临床相关危险因素的关系。方法:采用病例对照设计方法,选取124例PCa患者以及年龄、性别匹配的111例正常对照人群作为研究对象,使用聚合酶链反应-高分辨熔解曲线(PCR-HRM)技术结合测序验证法检测SNPrs10934853和rs2660753基因型与等位基因分布情况,分析两位点危险基因型的累积效应,并探讨其不同基因型与PCa患者临床相关危险因素间是否存在相关性。结果:①rs10934853的基因型AA、CC、AC在PCa组中分别有28例(22.8%)、46例(37.4%)、49例(39.8%),在正常对照组中分别有24例(22.0%)、34例(31.2%)、51例(46.8%);rs2660753的基因型AA、GG、AG在PCa组中分别有13例(11.0%)、59例(50.0%)、46例(39.0%),在正常对照组中分别有9例(8.8%)、47例(45.6%)、47例(45.6%),2个SNP的基因型频率与等位基因频率在2组间的分布均无显著性差异(P值分别为0.520、0.582)。②SNP rs10934853和rs2660753两独立变异危险基因型累积效应分析发现:与野生型组相比较,含有危险基因型的两组PCa的发病风险略有增加(OR值分别为1.831、1.968),但组间差异不具有统计学意义(P>0.05)。③SNP rs10934853和rs2660753的不同基因型与PCa患者不同临床相关危险因素间均无显著相关性(P>0.05)。结论:SNP rs10934853和rs2660753与中国PCa的发生无明显相关性,可能与中国PCa的发病风险无关。

Objective： To investigate the correlation of the common variant single nucleotide polymorphisms （SNP） on chromosome 3 with the incidence and related risk factors of prostate cancer （PCa） in Chinese men. Methods ： Using the case-control method, we included 124 PCa patients in the PCa group and 111 age- and gender-matched cancer-free heahhy subjects as normal controls. We detected the distribution of allele and genotype frequencies of the SNP rs10934853 and rs2660753 with the polymerase chain reaction-high resolution mehing curve （PCR-HRM） combined with gene sequencing, analyzed the cumulative effect of the risk genotypes of these two independent variants, and determined the correlation between different genotypes of these two SNPs and clinically related risk factors in the PCa patients. Results： As for the genotypes of rs10934853, there were 28 cases of AA （22.8%） , 46 cases of CC （37.4%）, and 49 cases of AC （39.8%） in the PCa patients, as compared with 24 （22.0%）, 34 （31.2%） and 51 （46.8%） in the healthy controls. As regards the genotypes of rs2660753, there were 13 cases of AA （11.0%）, 59 cases of GG （50.0%） and 46 cases of AG （39.0%） in the PCa patients, in comparison with 9 （8.8%）, 47 （45.6%） and 47 （45.6%） in the controls. No significant differences were found in the distribution of the genotype and allele frequencies of rs10934853 and rs2660753 between the two groups （P = 0.520 ＆ 0.582）. Analysis on the cumulative effect of the risk genotypes of rs10934853 and rs2660753 showed a slightly higher risk of PCa （ OR = 1. 831 ＆ 1. 968 ） in the two groups with risk genotypes than in the one with wild types （ P 〉 0.05 ）. Different genotypes of rs10934853 and rs2660753 were not correlated with clinically related risk factors of the PCa patients （P 〉 0.05 ）. Conclusion ： SNP rs10934853 and rs2660753 on chromosome 3 are not obviously correlated with PCa in Chinese patients, and may not be a genetic risk factor of PCa.