四君子汤对衰老小鼠脑组织caspase-3表达及活性的影响

Effects of Sijunzitang on expression and activity of caspase-3 in brain of aging mice

目的研究四君子汤对衰老小鼠脑组织caspase-3表达及活性的影响。方法雄性KM小鼠分为青年对照组、老年对照组和四君子汤组。四君子汤组每日灌胃四君子汤,其余两组动物灌胃等量蒸馏水,共8 w。应用免疫组化法检测海马caspase-3表达,分光光度法检测caspase-3酶活性。结果与青年对照组比较,老龄对照组小鼠海马caspase-3阳性细胞明显增多(P<0.05),脑组织caspase-3活性升高(P<0.01);与老龄对照组比较,四君子汤组海马caspase-3表达减少(P<0.05),脑caspase-3活性下降(P<0.01)。结论四君子汤可能减慢细胞凋亡速度延缓衰老。

Objective To investigate effects of Sijunzitang on expression and activity of caspase-3 in brain of aging mice.Methods Male KM mice were divided into 3 groups： young control,old control and Sijunzitang groups.Mice of Sijunzitang（SJZT） group were treated with intragastric administration of SJZT 12 g/kg,while mice of the other two groups were treated with distilled water.Immunohistochemical assay was used to detect the expression of caspase-3,and spectrophotometry was used to measure the activity of caspase-3.Results The expression and activity of caspase-3 were significantly increased in aging mice（P0.05,P0.01）.Compared with those in old control group,the expression and activity of caspase-3 of SJZG group were decreased significantly（P0.05,P0.01）.Conclusions SJZT could delay aging by slowing apoptosis.