益气活血法对脑出血大鼠脑组织Ⅳ型胶原表达的影响

Effect of method of supplementing Qi and activating blood circulation on the expression of type Ⅳ collagen in brains of intracerebral hemorrhage rats

目的探讨益气活血中药对脑出血大鼠脑损伤区组织Ⅳ型胶原表达的影响及其作用机制。方法 150只SD大鼠被随机分为假手术组、模型组、益气活血组、益气组和活血组各30只。用Ⅶ型胶原酶诱导脑出血大鼠模型。各组相应灌服蒸馏水、益气活血法代表方补阳还五汤全方及其益气组分、活血组分药物。用免疫组化方法检测术后3、71、42、1 d和28 dⅣ型胶原的表达,其结果进行平均光密度扫描半定量分析。结果假手术组不同时间点Ⅳ型胶原表达未见明显阳性信号;模型组、益气组、活血组及益气活血组脑出血后3 d即有Ⅳ型胶原表达,模型组及益气组至21 d达到高峰,随后开始下降;活血组及益气活血组表达高峰时间提前至14 d,并至21 d时仍维持一定的表达水平;各个时间点益气活血组表达均高于其他组。结论益气活血中药可能通过增强脑出血后血肿周围脑组织Ⅳ型胶原的表达参与组织修复。

Objective To investigate the mechanisms of method of supplementing Qi and activating blood circulation on the expression of type Ⅳ collagen in brains of intracerebral hemorrhage（ICH） rats.Methods ICH model was induced by injecting collagenase typeⅦ stereotaxically into right globus pallidus.One hundred and fifty SD rats were randomly divided into shame operation,ICH model,supplementing Qi,activating blood circulation,supplementing Qi and activating blood circulation groups,each group had 30 cases.The last 3 treated groups were administered orally with the classic methods of supplementing Qi and activating blood circulation prescription of Buyang Huanwu decoction.The expressions of type Ⅳ collagen were assayed by immunohistochemistry on 3,7,14,21 and 28 days after the onset,then the average optical density of the positive microvessels were counted.Results No hematoma and obvious local pathological changes were appeared in brain tissues of sham operation group at different time points during the experiment.Microvascular angiogenesis of supplementing Qi and activating blood circulation group was more than that of supplementing Qi,activating blood circulation and model groups.Immunohistochemical researches showed that at different time points,the expression of type Ⅳ collagen had no significant changes in sham operation group.The expressions of type Ⅳ collagen in model and three medically treated groups were observed on 3 day,the expressions were increased gradually,peaked on 21 day in supplementing Qi and model groups,and then started to decrease.The peak time of activating blood group and supplementing Qi and activating blood circulation group were advanced on 14 day,and the expressions sustained high levels to 28 day.Conclusions The method of supplementing Qi and activating blood circulation can up-regulate the expressions of type Ⅳ collagen after ICH to participate in the tissue repair.