血浆内皮素和一氧化氮以及血管内皮生长因子对2型糖尿病视网膜病变的影响

The effects of endothelin mitric oxide and vascular endothelial growth factor levels on thepatients with with type 2 diabetic retinopathy

目的探讨2型糖尿病视网膜病变（DR）患者血浆中内皮素（ET）、一氧化氮（NO）和血管内皮生长因子（VEGF）的变化。方法选择2型糖尿病患者80例，其中有视网膜病变53例（DR组），无视网膜病变27例（NDR组），另设健康对照者30名作为对照组。采用酶联免疫吸附双抗体夹心法测定其血浆ET和VEGF，硝酸还原法测定NO，并检测空腹血糖、餐后2h血糖、糖化血红蛋白、胆固醇、甘油三酯。结果DR组患者血浆ET为（80．68±13．57）mg／L，高于NDR组[（65．33±11．24）mg／L]及对照组[（45．25±9．06）mg／L]，差异均有统计学意义（p均〈0．01）；DR组NO为（69．82±14．89）μmol／L，明显高于对照组[（37．85±9．11）μmol／L]，但低于NDR组[（77．52±18．56）μmol／L]，差异均有统计学意义（P均〈0．05）；DR组VEGF[（110．52±25．65）μg/L]高于对照组[（82．42±18．47）μg/L]及NDR组[（97．55±25．61）μg/L]，差异均有统计学意义（JP均〈0．05）。结论ET、NO和VECF可能参与了2型糖尿病视网膜病变的发生与发展。

Obuective To study the changes of endothelin,nitric oxide and vascular endothelial growth factor level in patients with type 2 diabetic retinopathy （DR）. Methods Eighty diabetes patients （ 53 with diabetic retinopathy and 27 without）. Another 30 healthy volunteers were recruited as control. Plasma ET and VEGF levels were measured by enzyme-linked immunosorbent assay. NO levels were measured by nitrate reductase method. Results Plasma levels of El＇ were higher in patients with type 2 diabetes with DR （DR） （ 80. 68 ± 13.57 ） mg/L than （65.33 ± 11.24 ） mg/L, （ 45.25 ± 9. 06 ） mg/L, in control and in type 2 diabetes patieots without DR （NDR） （ Ps 〈 0, 01 ）, Plasma levels of NO in DR group （ 69. 82 ± 14. 89 ）umol/L were higher than（37.85 ±9. 11） umol/L, in control] group,but lower than （77. 52±18.56）umoL/L in NDR group （ Ps 〈 0. 05 ）. Plasma levels of VEGF （ 110.52 ±25.65 ） ±g/L in DR were significantly increased compared with -1 NDR（97.55±25.61）ug/L, （Ps〈0.05）.Conclusion ET, NO and control （82.42 ± 18.47）ug/L, and . VEGF may be involved in the pathogenesis of type 2 diabetic retinopathy.